Smirnova T A, Minenkova I B, Rebentish B A, Azizbekian R R
Mol Gen Mikrobiol Virusol. 1985 Jun(6):21-3.
Bacteriophages Tm2 and Tg27 of different origins but identical in biological properties have been compared. Physicochemical characteristics of bacteriophages have revealed the existence of end repeats and circular permutation of phage DNA. Phages Tm2 and Tg27 share the same dimensions of incapsulated DNA, differing in the sizes of phage genome and end repeats. Bacteriophage Tm2 genome is 45.2 kb. long with the end repeats containing 5.7%. The genome of Tg27 is 42.53 kb and 11.8% of end repeat. The bacteriophages relation has been confirmed by heteroduplex and restriction analysis. Tm2 and Tg27 share 84% of homology. Two regions of nonhomology are found representing a single-stranded loop and equishouldered vesicle with the sizes 2.19 kb and 5.03 kb, respectively.
已对来源不同但生物学特性相同的噬菌体Tm2和Tg27进行了比较。噬菌体的物理化学特性揭示了噬菌体DNA存在末端重复序列和环状排列。噬菌体Tm2和Tg27的包封DNA尺寸相同，但噬菌体基因组大小和末端重复序列不同。噬菌体Tm2基因组长度为45.2 kb，末端重复序列占5.7%。Tg27的基因组为42.53 kb，末端重复序列占11.8%。通过异源双链分析和限制性分析证实了噬菌体之间的关系。Tm2和Tg27具有84%的同源性。发现了两个非同源区域，分别代表大小为2.19 kb的单链环和5.03 kb的等肩泡。
