College of Horticulture, Hebei Agricultural University, Baoding 071001, Hebei, China.
College of Life Science, Hebei Agricultural University, Baoding 071001, Hebei, China.
Int J Biol Macromol. 2024 Apr;264(Pt 1):130429. doi: 10.1016/j.ijbiomac.2024.130429. Epub 2024 Feb 28.
CIPKs are kind of serine/threonine (Ser/Thr) protein kinases which play important roles in response to biotic and abiotic stresses, and in plant growth and development. However, CIPKs in jujube (Ziziphus jujuba Mill.) had limited information, especially regarding their response to cold stress. In the current study, a total of 18 ZjCIPKs were identified in jujube genome which unevenly distributed on seven chromosomes. Conserved motif and gene structural analysis depicted them with conserved DEGLSA and APE motifs and similar structures. Phylogenetic analysis indicated that CIPKs were classified into five subgroups (I-V). In addition, three pairs of ZjCIPKs exhibited tandem duplication while the segmental duplication of ZjCIPKs was not identified. Study on the cis-acting elements indicted that stress or hormone related cis-acting elements were distributed unevenly on ZjCIPKs promoters and most ZjCIPKs were down- or up-regulated by the cold stress. VIGS induced silencing of ZjCIPK5 decreased the cold tolerance of sour jujube. Subcellular location analysis showed ZjCIPK5 located in nucleus. Moreover, transcription factor ZjbHLH74 which was induced at 6 h under cold stress could interact with the promoter of ZjCIPK5 to regulate jujube cold tolerance. These findings provided insights to a molecular basis of CIPK5 in jujube cold tolerance breeding for future.
CIPKs 是一类丝氨酸/苏氨酸（Ser/Thr）蛋白激酶，在应对生物和非生物胁迫以及植物生长发育中发挥重要作用。然而，枣树（Ziziphus jujuba Mill.）中的 CIPKs 信息有限，尤其是它们对冷胁迫的响应。本研究在枣树基因组中鉴定出 18 个 ZjCIPKs，它们不均匀地分布在 7 条染色体上。保守基序和基因结构分析表明，它们具有保守的 DEGLSA 和 APE 基序和相似的结构。系统发育分析表明，CIPKs 分为五个亚组（I-V）。此外，有三对 ZjCIPKs 表现出串联重复，而 ZjCIPKs 的片段重复未被识别。顺式作用元件的研究表明，应激或激素相关的顺式作用元件在 ZjCIPKs 启动子上不均匀分布，大多数 ZjCIPKs 受冷胁迫的下调或上调。VIGS 诱导的 ZjCIPK5 沉默降低了酸枣的耐寒性。亚细胞定位分析表明 ZjCIPK5 位于细胞核内。此外，在冷胁迫下 6 小时诱导的转录因子 ZjbHLH74 可以与 ZjCIPK5 的启动子相互作用，调节枣树的耐寒性。这些发现为 CIPK5 在枣树耐寒性育种中的分子基础提供了新的见解。
