Inserm U1086 ANTICIPE (Interdisciplinary Research Unit for Cancer Prevention and Treatment), Normandie Univ, Université de Caen Normandie, Caen, France.
Comprehensive Cancer Center François Baclesse, UNICANCER, Caen, France.
Int Arch Occup Environ Health. 2024 May;97(4):353-363. doi: 10.1007/s00420-024-02049-z. Epub 2024 Mar 2.
Several studies investigated the link between agricultural occupational exposures and DNA damage, in an attempt to bring elements of biological plausibility to the increased cancer risk associated with them. However, only a few of these studies focused on females.
The comet assay was performed on PBMC (Peripheral Blood Mononuclear Cells) samples from 245 females working in open field farming and cattle raising, located in the Normandy area of France. Individual questionnaires on tasks performed were administered at the time of sampling to directly assess exposures. Environmental exposures were issued from a questionnaire assessing the farm productions. Linear regression analyses were done using the DNA damage scores.
Regarding direct exposures, several tasks associated with exposure to potentially harmful chemicals were not associated with DNA damage, but a longer duration of use of herbicide on meadows (p = 0.05) or of cleaning and upkeep of agricultural equipment (p = 0.06) revealed higher DNA damage levels, although the number of exposed women was low. Several indirect and/or environmental exposures were associated with DNA damage in multivariate analyses: a larger surface of meadows (p = 0.006) or the presence of poultry (p = 0.03) was associated with less DNA damage, while the presence of swine (p = 0.01) was associated with higher DNA damage. Smokers and former smokers had less DNA damage than non-smokers (p = 0.0008 and p = 0.03).
We report modified levels of DNA damage for those environmentally exposed to meadows, poultry and pig farming, underlining the need for a better knowledge of the potential health risks experienced by females in this setting.
多项研究调查了农业职业暴露与 DNA 损伤之间的联系，试图为与这些职业相关的癌症风险增加提供生物学上的合理性依据。然而，这些研究中只有少数关注女性。
对来自法国诺曼底地区从事露天农业和养牛工作的 245 名女性的 PBMC（外周血单核细胞）样本进行彗星试验。在采样时通过个人问卷评估所执行的任务，直接评估暴露情况。环境暴露情况来自评估农场生产的问卷。使用 DNA 损伤评分进行线性回归分析。
关于直接暴露，与接触潜在有害化学物质相关的几项任务与 DNA 损伤无关，但在草地上使用除草剂（p=0.05）或清洁和维护农业设备（p=0.06）的时间较长与更高的 DNA 损伤水平相关，尽管暴露的女性人数较少。在多变量分析中，几项间接和/或环境暴露与 DNA 损伤相关：更大的草地面积（p=0.006）或家禽的存在（p=0.03）与较少的 DNA 损伤相关，而猪的存在（p=0.01）与更高的 DNA 损伤相关。吸烟者和前吸烟者的 DNA 损伤少于非吸烟者（p=0.0008 和 p=0.03）。
我们报告了那些暴露于草地、家禽和养猪环境中的人 DNA 损伤程度的变化，这强调了需要更好地了解女性在这种环境下所面临的潜在健康风险。
