National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, China.
Hami-melon Research Center, Xinjiang Academy of Agricultural Sciences, Urumqi, 830091, China.
Plant J. 2024 Jun;118(6):2249-2268. doi: 10.1111/tpj.16705. Epub 2024 Mar 2.
Melon (Cucumis melo L.), being under intensive domestication and selective breeding, displays an abundant phenotypic diversity. Wild germplasm with tolerance to stress represents an untapped genetic resource for discovery of disease-resistance genes. To comprehensively characterize resistance genes in melon, we generate a telomere-to-telomere (T2T) and gap-free genome of wild melon accession PI511890 (C. melo var. chito) with a total length of 375.0 Mb and a contig N50 of 31.24 Mb. The complete genome allows us to dissect genome architecture and identify resistance gene analogs. We construct a pan-NLRome using seven melon genomes, which include 208 variable and 18 core nucleotide-binding leucine-rich repeat receptors (NLRs). Multiple disease-related transcriptome analyses indicate that most up-regulated NLRs induced by pathogens are shell or cloud NLRs. The T2T gap-free assembly and the pan-NLRome not only serve as essential resources for genomic studies and molecular breeding of melon but also provide insights into the genome architecture and NLR diversity.
甜瓜（Cucumis melo L.）经过集约化的驯化和选择性培育，表现出丰富的表型多样性。具有抗逆性的野生种质资源代表了一种未开发的遗传资源，可用于发现抗病基因。为了全面描述甜瓜中的抗性基因，我们生成了野生甜瓜品系 PI511890（C. melo var. chito）的端粒到端粒（T2T）和无间隙基因组，全长 375.0 Mb，串联群 N50 为 31.24 Mb。完整的基因组使我们能够剖析基因组结构并识别抗性基因类似物。我们使用七个甜瓜基因组构建了一个泛 NLRome，其中包括 208 个可变和 18 个核心核苷酸结合亮氨酸重复受体（NLRs）。多个与疾病相关的转录组分析表明，大多数由病原体诱导的上调 NLR 是壳或云 NLR。T2T 无间隙组装和泛 NLRome 不仅为甜瓜的基因组研究和分子育种提供了重要资源，而且还深入了解了基因组结构和 NLR 多样性。
