College of Ocean Food and Biological Engineering, Jimei University, Xiamen, Fujian 361021, People's Republic of China.
College of Ocean Food and Biological Engineering, Jimei University, Xiamen, Fujian 361021, People's Republic of China.
Food Chem. 2024 Jul 15;446:138892. doi: 10.1016/j.foodchem.2024.138892. Epub 2024 Feb 29.
Growing research confirms that lipid transport proteins play a key role in the trans-intestinal epithelial transport of carotenoids. In this study, to simultaneously improve the digestive stability and intestinal absorption of fucoxanthin (FX), functionalized vectors with a capability of up-regulating the expression of FX-specific lipid transporter proteins was fabricated. The results showed that myristic acid, palmitic acid, and stearic acid effectively promoted FX-specific lipid transporter protein expression and formed stable self-assembly complexes with Millard-modified zein (MZ). The FX was sufficiently encapsulated in the MZ-fatty acid (FA) particles, forming spherical nanoparticles with a "core-shell" structure. Simulated gastrointestinal digestion showed that FA introduction significantly increased the FX bioaccessibility. In vivo results further verified that adding FAs dramatically increased the FX serum response concentration. These findings suggest that incorporating nutrients that can promote lipid transporter protein expression into delivery vehicles should be an effective strategy for improving oral carotenoid absorption.
越来越多的研究证实，脂质转运蛋白在类胡萝卜素的跨肠上皮转运中起着关键作用。在这项研究中，为了同时提高叶黄素（FX）的消化稳定性和肠道吸收，制备了具有上调 FX 特异性脂质转运蛋白表达能力的功能化载体。结果表明，豆蔻酸、棕榈酸和硬脂酸能有效促进 FX 特异性脂质转运蛋白的表达，并与米尔拉德改性玉米醇溶蛋白（MZ）形成稳定的自组装复合物。FX 被充分包裹在 MZ-脂肪酸（FA）颗粒中，形成具有“核壳”结构的球形纳米颗粒。模拟胃肠道消化表明，FA 的引入显著增加了 FX 的生物利用度。体内结果进一步证实，添加 FA 可显著提高 FX 的血清反应浓度。这些发现表明，将能够促进脂质转运蛋白表达的营养素纳入递送载体应该是提高口服类胡萝卜素吸收的有效策略。
