Liu Yan, Wang Junli, Guo Jimin, Zhang Qianyi, Wang Shuqing, Hu Fen, Wu Jinghua, Zhao Yating, Zhang Jinghua, Yu Yuan, Li Yufeng, Zhang Xiaojun
College of Life Science, North China University of Science and Technology, Tangshan, 063210, China.
Hebei Key Laboratory of Molecular Oncology, Tangshan, 063001, China.
Sci Rep. 2024 Mar 4;14(1):5228. doi: 10.1038/s41598-024-56073-7.
BAZ2A, an epigenetic regulatory factor that affects ribosomal RNA transcription, has been shown to be highly expressed in several cancers and promotes tumor cell migration. This study explored the expression and mechanism of BAZ2A in tumorigenesis at the pan-cancer level. The Cancer Genome Atlas, Gene Expression Omnibus databases and TIMER2.0, cBioPortal and other tools were used to analyze the level of expression of BAZ2A in various tumor tissues and to examine the relationship between BAZ2A and survival, prognosis, mutation and immune invasion. In vitro experiments were performed to assess the function of BAZ2A in cancer cells. Using combined transcriptome and proteome analysis, we examined the possible mechanism of BAZ2A in tumors. BAZ2A exhibited high expression levels in multiple tumor tissues and displayed a significant association with cancer patient prognosis. The main type of BAZ2A genetic variation in cancer is gene mutation. Downregulation of BAZ2A inhibited proliferation, migration, and invasion and promoted apoptosis in LM6 liver cancer cell. The mechanism of BAZ2A in cancer development may involve lipid metabolism. These results help expand our understanding of BAZ2A in tumorigenesis and development and suggest BAZ2A may serve as a prognostic and diagnostic factor in several cancers.
BAZ2A是一种影响核糖体RNA转录的表观遗传调控因子，已被证明在多种癌症中高表达，并促进肿瘤细胞迁移。本研究在泛癌水平上探讨了BAZ2A在肿瘤发生中的表达及机制。利用癌症基因组图谱、基因表达综合数据库以及TIMER2.0、cBioPortal等工具，分析BAZ2A在各种肿瘤组织中的表达水平，并研究BAZ2A与生存、预后、突变及免疫浸润之间的关系。进行体外实验以评估BAZ2A在癌细胞中的功能。通过联合转录组和蛋白质组分析，我们研究了BAZ2A在肿瘤中的可能机制。BAZ2A在多种肿瘤组织中呈现高表达水平，且与癌症患者预后显著相关。癌症中BAZ2A基因变异的主要类型是基因突变。下调BAZ2A可抑制LM6肝癌细胞的增殖、迁移和侵袭，并促进其凋亡。BAZ2A在癌症发展中的机制可能涉及脂质代谢。这些结果有助于扩展我们对BAZ2A在肿瘤发生和发展中的理解，并表明BAZ2A可能作为多种癌症的预后和诊断因子。
