Ahmadi Akram Sadat, Shafiei-Jandaghi Nazanin Zahra, Sadeghi Kaveh, Nejati Ahmad, Zadheidar Sevrin, Mokhtari-Azad Talat, Yavarian Jila
Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Research Center for Antibiotic Stewardship and Antimicrobial Resistance, Tehran University of Medical Sciences, Tehran, Iran.
Iran J Public Health. 2023 Dec;52(12):2621-2629. doi: 10.18502/ijph.v52i12.14323.
Whole viral genome sequencing with next generation sequencing (NGS) technique is useful tool for determining the diversity of variants and mutations of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this study we have attempted to characterize the mutations and circulating variants of the SARSCoV-2 genome during the 4 wave of COVID-19 pandemic in Tehran, Iran in 2021.
We performed complete genome sequencing of 15 SARS-CoV-2 detected from 15 COVID-19 patients during the 4 wave of COVID-19 pandemic with NGS. Three groups of the patients at the beginning, middle and the end of the 4 wave were compared together.
We detected alpha and delta variants during the 4 wave of the pandemic. The results illustrated a dominance of amino acid substitution D614G in spike, and the most frequent mutants were N-R203K, G204R, S235F, nsp12-P323L, nsp6-G106del, G107del and F108del.
The detection of the virus mutations is a useful procedure for identifying the virus behavior and its genetic evolution in order to improve the efficacy of the monitoring strategies and therapeutic measures.
采用下一代测序（NGS）技术进行全病毒基因组测序是确定严重急性呼吸综合征冠状病毒2（SARS-CoV-2）变异体和突变多样性的有用工具。在本研究中，我们试图对2021年伊朗德黑兰新冠疫情第四波期间SARS-CoV-2基因组的突变和流行变异体进行特征分析。
我们对新冠疫情第四波期间从15例新冠肺炎患者中检测到的15株SARS-CoV-2进行了全基因组测序，采用的是NGS技术。将第四波开始、中期和末期的三组患者进行了比较。
我们在疫情第四波期间检测到了α和δ变异体。结果表明，刺突蛋白中氨基酸替代D614G占主导地位，最常见的突变体是N-R203K、G204R、S235F、nsp12-P323L、nsp6-G106del、G107del和F108del。
检测病毒突变是识别病毒行为及其基因进化的有用方法，有助于提高监测策略和治疗措施的有效性。
