Yaqoob Hafiza Sibgha, Shoaib Amna, Anwar Aneela, Perveen Shagufta, Javed Sidra, Mehnaz Samina
Department of Plant Pathology, Faculty of Agricultural Sciences, University of the Punjab, Lahore, Pakistan.
Department of Chemistry, University of Engineering and Technology, Lahore, Pakistan.
Physiol Mol Biol Plants. 2024 Jan;30(1):49-66. doi: 10.1007/s12298-023-01408-3. Epub 2024 Jan 9.
Seed bio-priming is a simple and friendly technique to improve stress resilience against fungal diseases in plants. An integrated approach of maize seeds biopriming with was applied in Zn-amended soil to observe the response against rot disease of (L.) caused by . Initially, the pathogen isolated from the infected corn was identified as based on morphology and sequences of the internally transcribed spacer region of the ribosomal RNA gene. Re-inoculation of maize seed with the isolated pathogen confirmed the pathogenicity of the fungus on the maize seeds. In vitro, the inhibitory potential of assessed on Zn-amended/un-amended growth medium revealed that antifungal potential of significantly improved in the Zn-amended medium, leading to 88% inhibition in fungal growth. Further assays with different concentrations (25, 50, and 75%) of cell pellet and the cultural filtrate of (with/without the Zn-amendment) showed a dose-dependent inhibitory effect on mycelial growth of the pathogen that also led to discoloration, fragmentation, and complete disintegration of the fungus hyphae and spores at 75% dose. In planta, biopriming of maize seeds with significantly managed disease, improved the growth and biochemical attributes (up to two-fold), and accelerated accumulation of lignin, polyphenols, and starch, especially in the presence of basal Zn. The results indicated that bioprimed seeds along with Zn as the most promising treatment for managing disease and improving plant growth traits through the enhanced accumulation of lignin, polyphenols, and starch, respectively.
种子生物引发是一种简单且友好的技术，可提高植物对真菌病害的抗逆性。在锌改良土壤中应用了玉米种子与[具体物质未给出]进行生物引发的综合方法，以观察对由[具体病原菌未给出]引起的玉米[具体病害未给出]的反应。最初，根据核糖体RNA基因内部转录间隔区的形态和序列，从感染玉米中分离出的病原菌被鉴定为[具体病原菌未给出]。用分离出的病原菌对玉米种子进行再接种，证实了该真菌对玉米种子的致病性。在体外，在锌改良/未改良生长培养基上评估[具体物质未给出]的抑制潜力，结果表明在锌改良培养基中[具体物质未给出]的抗真菌潜力显著提高，导致真菌生长抑制率达88%。进一步用不同浓度（25%、50%和75%）的[具体物质未给出]细胞沉淀和培养滤液（有/无锌改良）进行试验，结果显示对病原菌菌丝体生长有剂量依赖性抑制作用，在75%剂量时还导致真菌菌丝和孢子变色、破碎和完全解体。在植物体内，用[具体物质未给出]对玉米种子进行生物引发显著控制了病害，改善了生长和生化特性（提高了两倍），并加速了木质素、多酚和淀粉的积累，尤其是在有基础锌的情况下。结果表明，生物引发种子与锌结合是通过分别增强木质素、多酚和淀粉的积累来管理病害和改善植物生长性状的最有前景的处理方法。
