Jin Huang-Fei, Shi Ying, Jiao Yan-Hua, Cao Jun
College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou, 311121, China.
College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou, 311121, China.
Anal Chim Acta. 2024 Apr 8;1297:342359. doi: 10.1016/j.aca.2024.342359. Epub 2024 Feb 9.
Microemulsion electrokinetic chromatography (MEEKC) is a mode of capillary electrophoresis with a wide range of applications in which microemulsion is utilized as background electrolyte to achieve the separation of analytes. Microemulsions are composed of oil droplets, aqueous buffer, surfactant, and co-surfactant. Currently, conventional organic reagents act as the most commonly used oil phase in microemulsions, which are unfriendly to the environment. Recently, deep eutectic solvent (DES) has become a new type of eco-friendly solvent due to its non-toxicity. Therefore, it is of great value to establish a new MEEKC method by replacing conventional organic reagents as the oil phase with DES.
The novel DES/W MEEKC method was established for phenolic compounds in Senecio scandens samples. Single-factor experiments and response surface methodology were performed to systematically optimize the crucial parameters for the method, including the type and content of the oil phase, surfactant content, concentration of borax buffer, and pH of the background solution. Under the optimized conditions, satisfactory regression curves were established for all standard analytes with correlation coefficients ≥0.9990. The method featured high sensitivity and favorable accuracy, with the instrumental detection limit in the range of 0.22-1.04 μg/mL, and intraday and interday precision for migration time expressed as relative standard deviations of 0.18-0.82% and 1.25-2.50%, respectively. The DES/W MEEKC method was successfully applied to Senecio scandens with good recoveries of 87.72-106.99%. In conclusion, the newly established DES/W MEEKC method is highly efficient, green and environmentally friendly.
DES is considered a green and efficient solvent. The DES/W MEEKC method is highly efficient and environmentally friendly. Actually, the method provides a novel and effective analytical tool for the simultaneous separation and determination of multiple phenolic compounds, especially in complex plant matrices. In the future, the DES/W MEEKC method still has the prospect of being widely used in the separation of other complex phytochemicals.
微乳液电动色谱法（MEEKC）是一种毛细管电泳模式，具有广泛的应用，其中微乳液用作背景电解质以实现分析物的分离。微乳液由油滴、水性缓冲液、表面活性剂和助表面活性剂组成。目前，传统有机试剂是微乳液中最常用的油相，对环境不友好。近年来，深共熔溶剂（DES）因其无毒成为一种新型的环保型溶剂。因此，用DES替代传统有机试剂作为油相建立新的MEEKC方法具有重要价值。
建立了用于千里光样品中酚类化合物的新型DES/W MEEKC方法。进行了单因素实验和响应面法，以系统优化该方法的关键参数，包括油相的类型和含量、表面活性剂含量、硼砂缓冲液浓度和背景溶液的pH值。在优化条件下，所有标准分析物均建立了令人满意的回归曲线，相关系数≥0.9990。该方法具有高灵敏度和良好的准确性，仪器检测限在0.22 - 1.04μg/mL范围内，迁移时间的日内和日间精密度分别以相对标准偏差表示为0.18 - 0.82%和1.25 - 2.50%。DES/W MEEKC方法成功应用于千里光，回收率良好，为87.72 - 106.99%。总之，新建立的DES/W MEEKC方法高效、绿色且环保。
DES被认为是一种绿色高效的溶剂。DES/W MEEKC方法高效且环保。实际上，该方法为同时分离和测定多种酚类化合物提供了一种新颖有效的分析工具，特别是在复杂的植物基质中。未来，DES/W MEEKC方法在分离其他复杂植物化学物质方面仍有广泛应用的前景。
