College of Chemistry and Chemical Engineering, Liaoning Normal University, Huanghe Road 850#, Dalian, 116029, PR China.
Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, PR China.
Anal Chim Acta. 2024 Apr 8;1297:342379. doi: 10.1016/j.aca.2024.342379. Epub 2024 Feb 14.
Here, 1,3,4-thiadiazole unit was employed as novel excited state intramolecular proton transfer (ESIPT) structure to prepare favorable fluorescent probe. High selectivity and rapid response to Cu was obtained and the settling reaction was also used to recover ESIPT characteristics of probe to achieve sequential detection of HS. Remarkable color change of solution from colorless to bright yellow and fluorescence emission from green to dark realized the visual detection of Cu by naked eyes and transition of probe into portable fluorescent test strips. As expected, L-E could be utilized to quantitatively sense Cu and HS in different actual water and food samples including herbs, wine and fruits. The limits of detection for Cu and HS were as low as 34.5 nM and 38.6 nM. Also, probe L-E achieved real-time, portable, on-site quantitative detection of Cu via a colorimeter and a smartphone platform with limit of detection to 90.3 nM.
在这里，1,3,4-噻二唑单元被用作新型激发态分子内质子转移（ESIPT）结构，以制备有利的荧光探针。对 Cu 具有高选择性和快速响应，并且沉淀反应也用于恢复探针的 ESIPT 特性，从而实现对 HS 的顺序检测。溶液从无色到亮黄色和荧光发射从绿色到深黄色的明显颜色变化实现了 Cu 的肉眼可视化检测，以及探针转变为便携式荧光测试条。不出所料，L-E 可以用于定量检测不同实际水样和食品样品中的 Cu 和 HS，包括草药、葡萄酒和水果。Cu 和 HS 的检测限低至 34.5 nM 和 38.6 nM。此外，探针 L-E 通过光度计和智能手机平台实现了 Cu 的实时、便携式、现场定量检测，检测限低至 90.3 nM。
