Department of Analytical Chemistry, Faculty of Pharmacy, "Iuliu Haţieganu" University of Medicine and Pharmacy, 4 Pasteur Street, 400349, Cluj-Napoca, Romania.
Department of Analytical Chemistry, Faculty of Pharmacy, "Iuliu Haţieganu" University of Medicine and Pharmacy, 4 Pasteur Street, 400349, Cluj-Napoca, Romania; Departamento de Química Física y Analítica, Universidad de Oviedo, Av. Julián Clavería 8, 33006, Oviedo, Spain.
Anal Chim Acta. 2024 Apr 8;1297:342325. doi: 10.1016/j.aca.2024.342325. Epub 2024 Feb 3.
This review summarizes the stepwise strategy and key points for magnetic beads (MBs)-based aptamer selection which is suitable for isolating aptamers against small and large molecules via systematic evolution of ligands by exponential enrichment (SELEX). Particularities, if any, are discussed according to the target size. Examples targeting small molecules (<1000 Da) such as xenobiotics, toxins, pesticides, herbicides, illegal additives, hormones, and large targets such as proteins (biomarkers, pathogens) are discussed and presented in tabular formats. Of special interest are the latest advances in more efficient alternatives, which are based on novel instrumentation, materials or microelectronics, such as fluorescence MBs-SELEX or microfluidic chip system-assisted MBs-SELEX. Limitations and perspectives of MBs-SELEX are also reviewed. Taken together, this review aims to provide practical insights into MBs-SELEX technologies and their ability to screen multiple potential aptamers against targets from small to large molecules.
本文综述了基于磁珠（MBs）的适体筛选的逐步策略和要点，该策略适合通过指数富集配体系统进化（SELEX）从小分子和大分子中分离适体。根据目标大小讨论了任何特殊性。讨论并以表格形式呈现了针对小分子（<1000 Da）如外源性化合物、毒素、杀虫剂、除草剂、非法添加剂、激素和大分子如蛋白质（生物标志物、病原体）的适体的示例。特别感兴趣的是基于新型仪器、材料或微电子学的更有效的替代方法的最新进展，例如荧光 MBs-SELEX 或微流控芯片系统辅助 MBs-SELEX。还综述了 MBs-SELEX 的局限性和展望。总的来说，本文旨在为 MBs-SELEX 技术及其筛选从小分子到大分子的多个潜在适体的能力提供实用的见解。
