Bibani Malek, Casian Magdolna, Feier Bogdan, Bogdan Diana, Hosu-Stancioiu Oana, Ktari Nadia, Kalfat Rafik, Cristea Cecilia
Department of Analytical Chemistry, Faculty of Pharmacy, "Iuliu Hațieganu" University of Medicine and Pharmacy, 4 Pasteur Street, 400349, Cluj-Napoca, Romania.
Laboratoire Matériaux, Traitement Et Analyse, INRAP, BiotechPole Sidi-Thabet, 2020, Ariana, Tunisia.
Mikrochim Acta. 2025 Jan 25;192(2):107. doi: 10.1007/s00604-025-06952-1.
A label-free, flexible, and disposable aptasensor was designed for the rapid on-site detection of vancomycin (VAN) levels. The electrochemical sensor was based on lab-printed carbon electrodes (C-PE) enriched with cauliflower-shaped gold nanostructures (AuNSs), on which VAN-specific aptamers were immobilized as biorecognition elements and short-chain thiols as blocking agents. The AuNSs, characterized by scanning electron microscopy (SEM) and atomic force microscopy (AFM), enhanced the electrochemical properties of the platform and the aptamer immobilization active sites. The developed disposable aptasensor allowed label-free detection of VAN via electrochemical impedance spectroscopy (EIS) across a wide range of concentrations (50-1000 nM), with a limit of detection (LOD) of 1.721 nM. The aptasensor presented good selectivity against some commonly found interferences in human serum and milk and was successfully applied to the analysis of these samples.
设计了一种无标记、灵活且一次性使用的适体传感器，用于快速现场检测万古霉素（VAN）水平。该电化学传感器基于实验室打印的富含菜花状金纳米结构（AuNSs）的碳电极（C-PE），在其上固定了VAN特异性适体作为生物识别元件，并使用短链硫醇作为封闭剂。通过扫描电子显微镜（SEM）和原子力显微镜（AFM）表征的AuNSs增强了平台的电化学性能和适体固定活性位点。所开发的一次性适体传感器能够通过电化学阻抗谱（EIS）在宽浓度范围（50 - 1000 nM）内对VAN进行无标记检测，检测限（LOD）为1.721 nM。该适体传感器对人血清和牛奶中一些常见干扰物具有良好的选择性，并成功应用于这些样品的分析。
