全基因组鉴定和烟草（Nicotiana tabacum L.）NF-Y 基因家族的表达分析。

Genome-wide identification and expression analysis of NF-Y gene family in tobacco (Nicotiana tabacum L.).

机构信息

College of Plant Protection and Agricultural Big-Data Research Center, Shandong Agricultural University, Tai'an, 271018, China.

State Forestry and Grassland Administration Key Laboratory of Silviculture in Downstream Areas of the Yellow River, College of Forestry, Shandong Agricultural University, Tai'an, China.

出版信息

Sci Rep. 2024 Mar 4;14(1):5257. doi: 10.1038/s41598-024-55799-8.

DOI:10.1038/s41598-024-55799-8

PMID:38438470

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10912202/

Abstract

Nuclear factor Y (NF-Y) gene family is an important transcription factor composed of three subfamilies of NF-YA, NF-YB and NF-YC, which is involved in plant growth, development and stress response. In this study, 63 tobacco NF-Y genes (NtNF-Ys) were identified in Nicotiana tabacum L., including 17 NtNF-YAs, 30 NtNF-YBs and 16 NtNF-YCs. Phylogenetic analysis revealed ten pairs of orthologues from tomato and tobacco and 25 pairs of paralogues from tobacco. The gene structure of NtNF-YAs exhibited similarities, whereas the gene structure of NtNF-YBs and NtNF-YCs displayed significant differences. The NtNF-Ys of the same subfamily exhibited a consistent distribution of motifs and protein 3D structure. The protein interaction network revealed that NtNF-YC12 and NtNF-YC5 exhibited the highest connectivity. Many cis-acting elements related to light, stress and hormone response were found in the promoter of NtNF-Ys. Transcriptome analysis showed that more than half of the NtNF-Y genes were expressed in all tissues, and NtNF-YB9/B14/B15/B16/B17/B29 were specifically expressed in roots. A total of 15, 12, 5, and 6 NtNF-Y genes were found to respond to cold, drought, salt, and alkali stresses, respectively. The results of this study will lay a foundation for further study of NF-Y genes in tobacco and other Solanaceae plants.

摘要

核因子 Y（NF-Y）基因家族是一个重要的转录因子，由 NF-YA、NF-YB 和 NF-YC 三个亚家族组成，参与植物的生长、发育和应激反应。本研究在烟草中鉴定了 63 个 NF-Y 基因（NtNF-Ys），包括 17 个 NtNF-YA、30 个 NtNF-YB 和 16 个 NtNF-YC。系统进化分析表明，番茄和烟草中有 10 对直系同源基因，烟草中有 25 对旁系同源基因。NtNF-YA 的基因结构具有相似性，而 NtNF-YB 和 NtNF-YC 的基因结构则存在显著差异。同一亚家族的 NtNF-Ys 具有一致的基序和蛋白质 3D 结构分布。蛋白互作网络显示，NtNF-YC12 和 NtNF-YC5 具有最高的连接性。在 NtNF-Ys 的启动子中发现了许多与光、应激和激素响应相关的顺式作用元件。转录组分析表明，超过一半的 NtNF-Y 基因在所有组织中表达，而 NtNF-YB9/B14/B15/B16/B17/B29 则特异性表达于根中。共有 15、12、5 和 6 个 NtNF-Y 基因分别对冷、干旱、盐和碱胁迫有响应。本研究结果将为进一步研究烟草和其他茄科植物的 NF-Y 基因奠定基础。