单细胞 RNA 测序揭示与人类视神经脊髓炎谱系障碍相关的细胞类型特异性免疫调节。
Single-cell RNA sequencing reveals cell type-specific immune regulation associated with human neuromyelitis optica spectrum disorder.
机构信息
Department of Neurology, Henan Joint International Research Laboratory Of Accurate Diagnosis, Treatment, Research And Development, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou, Henan, China.
Department of Neurology, Zhoukou Central Hospital, Zhoukou, Henan, China.
出版信息
Front Immunol. 2024 Feb 19;15:1322125. doi: 10.3389/fimmu.2024.1322125. eCollection 2024.
INTRODUCTION
One rare type of autoimmune disease is called neuromyelitis optica spectrum disorder (NMOSD) and the peripheral immune characteristics of NMOSD remain unclear.
METHODS
Here, single-cell RNA sequencing (scRNA-seq) is used to characterize peripheral blood mononuclear cells from individuals with NMOSD.
RESULTS
The differentiation and activation of lymphocytes, expansion of myeloid cells, and an excessive inflammatory response in innate immunity are observed. Flow cytometry analyses confirm a significant increase in the percentage of plasma cells among B cells in NMOSD. NMOSD patients exhibit an elevated percentage of CD8+ T cells within the T cell population. Oligoclonal expansions of B cell receptors are observed after therapy. Additionally, individuals with NMOSD exhibit elevated expression of CXCL8, IL7, IL18, TNFSF13, IFNG, and NLRP3.
DISCUSSION
Peripheral immune response high-dimensional single-cell profiling identifies immune cell subsets specific to a certain disease and identifies possible new targets for NMOSD.
简介
一种罕见的自身免疫性疾病称为视神经脊髓炎谱系疾病（NMOSD），其外周免疫特征仍不清楚。
方法
本研究采用单细胞 RNA 测序（scRNA-seq）技术对 NMOSD 患者的外周血单个核细胞进行特征分析。
结果
观察到淋巴细胞的分化和激活、髓样细胞的扩增以及固有免疫的过度炎症反应。流式细胞术分析证实 NMOSD 患者 B 细胞中浆细胞的比例显著增加。NMOSD 患者 T 细胞群中 CD8+T 细胞的比例升高。治疗后观察到 B 细胞受体的寡克隆扩增。此外，NMOSD 患者表达 CXCL8、IL7、IL18、TNFSF13、IFNG 和 NLRP3 的水平升高。
讨论
外周免疫反应的高维单细胞分析确定了特定疾病的免疫细胞亚群，并为 NMOSD 确定了可能的新靶点。
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