Department of Gastroenterology, The Third Clinical Institute Affiliated to Wenzhou Medical University, Wenzhou People's Hospital, Wenzhou325035, China.
Department of Scientific Research Center, The Third Clinical Institute Affiliated to Wenzhou Medical University, Wenzhou People's Hospital, Wenzhou Maternal and Child Health Care Hospital, Wenzhou325035, China.
Food Funct. 2024 Mar 18;15(6):3199-3213. doi: 10.1039/d3fo05221d.
Ageing is defined as the degeneration of physiological functions in numerous tissues and organs of an organism, which occurs with age. As we age, the gut undergoes a series of changes and weaknesses that may contribute to overall ageing. Emerging evidence suggests that β-nicotinamide mononucleotide (NMN) plays a role in regulating intestinal function, but there is still a lack of literature on its role in maintaining the colon health of ageing mice. In our research, mice proved that NMN prolonged their life span and delayed senescence. This study was designed to investigate the effects of long-term intervention on regulating colon function in ageing mice. Our results indicated that NMN improved the pathology of intestinal epithelial cells and intestinal permeability by upregulating the expression of intestinal tight junction proteins and the number of goblet cells, increasing the release of anti-inflammatory factors, and increasing beneficial intestinal bacteria. NMN increased the expression of the proteins SIRT1, NMNAT2, and NMNAT3 and decreased the expression of the protein P53. It also regulated the activity of ISCs by increasing Wnt/β-catenin and Lgr5. Our findings also revealed that NMN caused a significant increase in the relative abundance of and and notable differences in metabolic pathways related to choline metabolism in cancer. In summary, NMN supplementation can delay frailty in old age, aid healthy ageing, and delay gut ageing.
衰老是指生物体众多组织和器官的生理功能随年龄增长而退化。随着年龄的增长，肠道会经历一系列变化和衰弱，这可能导致整体衰老。新出现的证据表明，β-烟酰胺单核苷酸（NMN）在调节肠道功能方面发挥作用，但关于其在维持衰老小鼠结肠健康方面的作用，文献仍然缺乏。在我们的研究中，证实 NMN 延长了小鼠的寿命并延缓了衰老。本研究旨在探讨长期干预对调节衰老小鼠结肠功能的影响。我们的结果表明，NMN 通过上调肠道紧密连接蛋白的表达和杯状细胞的数量，增加抗炎因子的释放，增加有益的肠道细菌，改善肠道上皮细胞和肠道通透性。NMN 增加了 SIRT1、NMNAT2 和 NMNAT3 蛋白的表达，降低了 P53 蛋白的表达。它还通过增加 Wnt/β-catenin 和 Lgr5 来调节 ISC 的活性。我们的研究结果还表明，NMN 导致丰度显著增加和显著增加与胆碱代谢相关的癌症代谢途径的差异。总之，NMN 补充剂可以延缓老年虚弱，促进健康衰老，延缓肠道衰老。
