School and Hospital of Stomatology, Tianjin Medical University, Tianjin 30070, China.
Zhongnuo Dental Hospital, Tianjin Nankai District, Tianjin 300101, China.
ACS Biomater Sci Eng. 2024 Apr 8;10(4):2463-2476. doi: 10.1021/acsbiomaterials.3c01858. Epub 2024 Mar 6.
The challenges in the treatment of extensive bone defects are infection control and bone regeneration. Bone tissue engineering is currently one of the most promising strategies. In this study, a short biopeptide with specific osteogenic ability is designed by fusion peptide technology and encapsulated with chitosan-modified poly(lactic acid-glycolic acid) (PLGA) microspheres. The fusion peptide (FP) mainly consists of an osteogenic functional sequence (P-15) and a bone-specific binding sequence (Asp-6), which can regulate bone formation accurately and efficiently. Chitosan-modified PLGA with antimicrobial and pro-healing effects is used to achieve the sustained release of fusion peptides. In the early stage, the antimicrobial and soft tissue healing effects can stop the wound infection as soon as possible, which is relevant for the subsequent bone regeneration process. Our data show that CS-PLGA@FP microspheres have antibacterial and pro-cell migration effects in vitro and excellent pro-wound-healing effects in vivo. In addition, CS-PLGA@FP microspheres promote the expression of osteogenic-related factors and show excellent bone regeneration in a rat defect model. Therefore, CS-PLGA@FP microspheres are an efficient biomaterial that can accelerate the recovery of bone defects.
治疗广泛骨缺损的挑战在于感染控制和骨再生。骨组织工程是目前最有前途的策略之一。在这项研究中，通过融合肽技术设计了一种具有特定成骨能力的短生物肽，并将其封装在壳聚糖修饰的聚乳酸-羟基乙酸共聚物（PLGA）微球中。融合肽（FP）主要由成骨功能序列（P-15）和骨特异性结合序列（Asp-6）组成，能够精确高效地调节骨形成。具有抗菌和促愈合作用的壳聚糖修饰的 PLGA 用于实现融合肽的持续释放。在早期，具有抗菌和软组织愈合作用的壳聚糖修饰的 PLGA 能够尽快阻止伤口感染，这对于随后的骨再生过程至关重要。我们的数据表明，CS-PLGA@FP 微球在体外具有抗菌和促进细胞迁移的作用，在体内具有优异的促进伤口愈合的作用。此外，CS-PLGA@FP 微球能够促进成骨相关因子的表达，并在大鼠缺损模型中表现出优异的骨再生能力。因此，CS-PLGA@FP 微球是一种能够加速骨缺损恢复的高效生物材料。
