含抗菌肽 OP-145 的 PLGA 微球对骨感染中临床分离病原体的抗菌作用。
The antimicrobial effects of PLGA microspheres containing the antimicrobial peptide OP-145 on clinically isolated pathogens in bone infections.
机构信息
Department of Orthopaedics, Xinhua Hospital of Zhejiang Province, The Second Affiliated Hospital of Zhejiang Chinese Medical University, No. 318 Chaowang Road, Gongshu District, Hangzhou, 310003, Zhejiang, China.
Hangzhou Plastic Surgery Hospital, Hangzhou, China.
出版信息
Sci Rep. 2022 Aug 25;12(1):14541. doi: 10.1038/s41598-022-18690-y.
Infection after fracture is a significant problem for the healing of fractures. Antimicrobial peptides combined with PLGA (poly-lactic-co-glycolic acid) microspheres can open new horizons for treating bone infections. Twenty rats in the control group were treated with physiologic saline solution after surgery, and 20 rats in the treatment group were treated with OP-145 PLGA microspheres and vancomycin after surgery. The biofilms from treatment and control groups were analyzed by fluorescence microscopy. Blood samples were collected at 12, 24, 36, 48, and 72 h. OP-145 PLGA microspheres showed significant inhibitory effects on clinically isolated strains (P < 0.05) and there were significant differences in serum CRP (P < 0.05) levels compared with control group. In conclusion, OP-145 PLGA microspheres could slowly release antimicrobial peptides and significantly reduce biofilm formation and levels of inflammatory factors.
骨折感染是骨折愈合的一个重大问题。抗菌肽与 PLGA（聚乳酸-羟基乙酸共聚物）微球的结合为治疗骨感染开辟了新的前景。对照组 20 只大鼠在手术后用生理盐水治疗，治疗组 20 只大鼠在手术后用 OP-145 PLGA 微球和万古霉素治疗。荧光显微镜分析治疗组和对照组的生物膜。在 12、24、36、48 和 72 小时采集血液样本。OP-145 PLGA 微球对临床分离株有显著的抑制作用（P<0.05），与对照组相比，血清 CRP 水平有显著差异（P<0.05）。总之，OP-145 PLGA 微球可以缓慢释放抗菌肽，显著减少生物膜的形成和炎症因子的水平。
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