Institute of Biomedical Chemistry, Moscow, Russia.
Biomed Khim. 2024 Feb;70(1):61-68. doi: 10.18097/PBMC20247001061.
Using the method of shotgun mass spectrometry, we have evaluated changes in the proteomic profile of HaCat cells in response to the treatment with sodium dodecyl sulfate (anionic surfactant) and Triton-X100 (non-ionic surfactant) in two concentrations (12.5 µg/ml and 25.0 µg/ml). The study revealed induction of orphan CYP2S1 (biotransformation phase I) in response to Triton-X100. We have identified proteins of II (glutathione-S-transferases, GSTs) and III (solute carrier proteins, SLCs) biotransformation phases, as well as antioxidant proteins (peroxiredoxins, PRDXs; catalase, CAT; thioredoxin, TXN). Thus, proteins of all three xenobiotic detoxification phases were detected. The presented results suggest a new prospect of using HaCaT keratinocytes as a model of human epidermis for studying the metabolism of drugs/toxicants in human skin in vitro.
采用“鸟枪法”质谱技术，我们评估了 HaCat 细胞在受到十二烷基硫酸钠（阴离子表面活性剂）和 Triton-X100（非离子表面活性剂）两种浓度（12.5 µg/ml 和 25.0 µg/ml）处理后的蛋白质组图谱变化。研究结果表明，Triton-X100 可诱导孤儿 CYP2S1（生物转化 I 期）。我们鉴定出了生物转化 II 期（谷胱甘肽-S-转移酶，GSTs）和 III 期（溶质载体蛋白，SLCs）以及抗氧化蛋白（过氧化物还原酶，PRDXs；过氧化氢酶，CAT；硫氧还蛋白，TXN）的蛋白。因此，检测到了所有三个外来物质解毒阶段的蛋白。这些结果表明，使用 HaCaT 角质形成细胞作为人表皮模型，在体外研究人皮肤中药物/毒物的代谢具有新的前景。
