Rusanov A L, Petushkova N A, Poverennaya E V, Nakhod K V, Larina O V, Lisitsa A V, Luzgina N G
Research and Manufacturing Association "Perspectiva", Moscow, Russia.
Institute of Biomedical Chemistry, Moscow, Russia.
Biomed Khim. 2017 Oct;63(5):405-412. doi: 10.18097/PBMC20176305405.
The effects of sodium dodecyl sulfate (25 mg/ml) and Triton X-100 (12.5 mg/ml and 25 mg/ml) on the HaCaT immortalized keratinocytes exposed to these surfactants for 48 h were studied. Using shotgun proteomics, a comparative analysis of the proteomic profiles of control and experimental cells after surfactants exposure was carried out. 260 common proteins were identified in control and experimental cells; 33 proteins were found in cells exposed to all three treatments, but not in control cells. These 33 proteins apparently reflect a nonspecific (universal) response of cells to toxic damage by the surfactants. These proteins are associated with activation of cell proliferation, changes in the functional activity of their ER and mitochondria, increased mRNA stability and activation of protein degradation processes in the cells. The possibility of using these proteins as a nonspecific parameter of cell response to cytotoxic damage is discussed. The mass spectrometry proteomics data ("raw", "mgf" and "xml" files) have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifiers PXD007789 and PXD007776.
研究了十二烷基硫酸钠（25毫克/毫升）和曲拉通X-100（12.5毫克/毫升和25毫克/毫升）对暴露于这些表面活性剂48小时的HaCaT永生化角质形成细胞的影响。采用鸟枪法蛋白质组学，对表面活性剂暴露后对照细胞和实验细胞的蛋白质组图谱进行了比较分析。在对照细胞和实验细胞中鉴定出260种常见蛋白质；在所有三种处理的细胞中发现了33种蛋白质，但在对照细胞中未发现。这33种蛋白质显然反映了细胞对表面活性剂毒性损伤的非特异性（普遍）反应。这些蛋白质与细胞增殖的激活、内质网和线粒体功能活性的变化、mRNA稳定性的增加以及细胞内蛋白质降解过程的激活有关。讨论了将这些蛋白质用作细胞对细胞毒性损伤反应的非特异性参数的可能性。质谱蛋白质组学数据（“原始”、“mgf”和“xml”文件）已通过PRIDE合作伙伴库存入蛋白质组交换联盟，数据集标识符为PXD007789和PXD007776。
