Kisrieva Y S, Samenkova N F, Larina O B, Zgoda V G, Karuzina I I, Rusanov A L, Luzgina N G, Petushkova N A
Institute of Biomedical Chemistry, Moscow, Russia.
LLC "Perspective", Novosibirsk, Russia.
Biomed Khim. 2020 Nov;66(6):469-476. doi: 10.18097/PBMC20206606469.
Using electrospray ionization tandem mass spectrometry, a comparative analysis of the HaCaT keratinocyte proteins encoded by the 18th chromosome was performed before and after exposure to sodium dodecyl sulfate (25 mg/ml) and to Triton X-100 (12.5 mg/ml) in a subtoxic dose for 48 hours. Proteins were identified using the SearchGUI platform (X!Tandem and MS-GF+ search engines). In total, 1284 proteins were found in immortalized human HaCaT keratinocytes and about 75% of them were identified by two or more peptides. Were identified, that 26 proteins were encoded by genes of chromosome 18. Among these proteins, 17 were common for control cells and HaCaT cells treated with SDS. Proteins MARE2 and CTIF were identified only in control keratinocytes. Seven identified proteins encoded by genes of chromosome 18 were found only in detergent-treated keratinocytes: LMAN1, NDUV2, SPB3, VPS4B, KDSR, ROCK1 and RHG28.
使用电喷雾电离串联质谱法，对永生化人HaCaT角质形成细胞在暴露于十二烷基硫酸钠（25mg/ml）和亚毒性剂量的吐温X-100（12.5mg/ml）48小时之前和之后由第18号染色体编码的蛋白质进行了比较分析。使用SearchGUI平台（X!Tandem和MS-GF+搜索引擎）鉴定蛋白质。在永生化人HaCaT角质形成细胞中共发现1284种蛋白质，其中约75%由两种或更多种肽段鉴定出来。已鉴定出26种蛋白质由第18号染色体的基因编码。在这些蛋白质中，17种在对照细胞和用SDS处理的HaCaT细胞中是共有的。蛋白质MARE2和CTIF仅在对照角质形成细胞中被鉴定出来。在仅经去污剂处理的角质形成细胞中发现了7种由第18号染色体基因编码的已鉴定蛋白质：LMAN1、NDUV2、SPB3、VPS4B、KDSR、ROCK1和RHG28。
