Rusanov A L, Nakhod K V, Nakhod V I, Poverennaya E V, Petushkova N A, Luzgina N G
Perspective Company, Novosibirsk, Russia.
V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia.
Bull Exp Biol Med. 2017 Sep;163(5):620-622. doi: 10.1007/s10517-017-3863-2. Epub 2017 Sep 27.
Changes in the proteome of keratinocytes of immortalized HaCaT line exposed to cytotoxic substance Triton X-100 in concentrations of 12.5 and 25 μg/ml were studied by liquid chromatography combined with mass spectrometry. The appearance of proteins involved in the regulation of mitosis, RNA stability, and catabolic processes were detected; the number of apoptosis-associated proteins increased, while the number of proteins involved in differentiation and energy metabolism of keratinocytes decreased.
通过液相色谱与质谱联用技术，研究了永生化HaCaT细胞系角质形成细胞蛋白质组在浓度为12.5和25μg/ml的细胞毒性物质Triton X-100作用下的变化。检测到参与有丝分裂调控、RNA稳定性和分解代谢过程的蛋白质出现；凋亡相关蛋白数量增加，而参与角质形成细胞分化和能量代谢的蛋白质数量减少。
