State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, 150069, China.
Heilongjiang Veterinary Biopharmaceutical Engineering Technology Research Center, Harbin, 150069, China.
Arch Virol. 2024 Mar 7;169(3):67. doi: 10.1007/s00705-024-05997-6.
Porcine circovirus type 4 (PCV4), first identified in 2019 as a newly emerging pathogen, has been found in several provinces of China, as well as in Korea and Thailand. Since PCV4 is not included in immunization programs, epidemiological investigations should be conducted for detection of anti-PCV4 antibodies. Virus-like particles (VLPs) are frequently used for serological analysis of pathogen infections. However, there have been no reports on using PCV4 VLPs for serological investigation of PCV4 infection. In this study, we generated self-assembled PCV4 VLPs using an E. coli expression system, purified them using a two-step process, and used them to develop an indirect ELISA. This ELISA method was found to be highly specific, sensitive, and repeatable, making it suitable for PCV4 antibody detection in serum samples. Finally, the ELISA was used to analyze 422 serum samples collected from across several regions in China, 134 of which tested positive. Thus, the PCV4-VLP-based ELISA can effectively detect antibodies against PCV4 in serum samples, making it a useful tool for PCV4 epidemiology.
猪圆环病毒 4 型（PCV4）于 2019 年首次被鉴定为一种新出现的病原体，已在中国多个省份以及韩国和泰国发现。由于 PCV4 未包含在免疫计划中，因此应进行流行病学调查以检测抗 PCV4 抗体。病毒样颗粒（VLPs）常用于病原体感染的血清学分析。然而，目前尚无关于使用 PCV4 VLPs 进行 PCV4 感染血清学研究的报道。在本研究中，我们使用大肠杆菌表达系统生成了自组装的 PCV4 VLPs，通过两步法对其进行纯化，并使用它们开发了间接 ELISA。该 ELISA 方法具有高度特异性、敏感性和可重复性，适用于血清样品中 PCV4 抗体的检测。最后，该 ELISA 用于分析了来自中国多个地区的 422 份血清样本，其中 134 份为阳性。因此，基于 PCV4-VLP 的 ELISA 可有效检测血清样品中针对 PCV4 的抗体，是一种用于 PCV4 流行病学研究的有用工具。
