Department of Orthopedic Surgery, Social insurance Tagawa Hospital, 10-18 kamihon-machi, Tagawa, Fukuoka 826-0023, Japan.
Department of Orthopedic Surgery, Kurume University Medical Center, 151-1 Kokubu-machi, Kurume, Fukuoka 839-0863, Japan.
J Int Med Res. 2024 Mar;52(3):3000605241232550. doi: 10.1177/03000605241232550.
To investigate the effect of adipose-derived cells (ADCs) on tendon-bone healing in a rat model of chronic rotator cuff tear (RCT) with suprascapular nerve (SN) injury.
Adult rats underwent right shoulder surgery whereby the supraspinatus was detached, and SN injury was induced. ADCs were cultured from the animals' abdominal fat. At 6 weeks post-surgery, the animals underwent surgical tendon repair; the ADC (+ve) group (n = 18) received an ADC injection, and the ADC (-ve) group (n = 18) received a saline injection. Shoulders were harvested at 10, 14, and 18 weeks and underwent histological, fluorescent, and biomechanical analyses.
In the ADC (+ve) group, a firm enthesis, including dense mature fibrocartilage and well-aligned cells, were observed in the bone-tendon junction and fatty infiltration was less than in the ADC (-ve) group. Mean maximum stress and linear stiffness was greater in the ADC (+ve) compared with the ADC (-ve) group at 18 weeks.
ADC supplementation showed a positive effect on tendon-bone healing in a rat model of chronic RCT with accompanying SN injury. Therefore, ADC injection may possibly accelerate recovery in massive RCT injuries.
研究脂肪来源细胞（ADCs）对伴有肩胛上神经（SN）损伤的慢性肩袖撕裂（RCT）大鼠模型中腱骨愈合的影响。
成年大鼠接受右肩手术，使冈上肌分离，并诱导 SN 损伤。从动物腹部脂肪中培养 ADCs。术后 6 周，动物接受手术肌腱修复；ADCs（+ve）组（n=18）接受 ADC 注射，ADCs（-ve）组（n=18）接受生理盐水注射。在第 10、14 和 18 周时采集肩部进行组织学、荧光和生物力学分析。
在 ADC（+ve）组中，在骨腱交界处观察到坚固的附着点，包括密集的成熟纤维软骨和排列整齐的细胞，脂肪浸润少于 ADC（-ve）组。与 ADC（-ve）组相比，在 18 周时，ADCs（+ve）组的最大应力和线性刚度均较大。
ADCs 补充对伴有 SN 损伤的慢性 RCT 大鼠模型中的腱骨愈合有积极影响。因此，ADCs 注射可能有助于加速巨大 RCT 损伤的恢复。
