School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang, 212013, People's Republic of China.
Extremophiles. 2024 Jun 18;28(2):28. doi: 10.1007/s00792-024-01347-1.
Four halophilic archaeal strains YCN1, YCN58, LT38, and LT62 were isolated from Yuncheng Salt Lake (Shanxi, China) and Tarim Basin (Xinjiang, China), respectively. Phylogenetic and phylogenomic analyses showed that these four strains tightly cluster with related species of Halobacterium, Natronomonas, Halorientalis, and Halobellus, respectively. The AAI, ANI, and dDDH values between these four strains and their related species of respective genera were lower than the proposed threshold values for species delineation. Strains YCN1, YCN58, LT38, and LT62 could be differentiated from the current species of Halobacterium, Natronomonas, Halorientalis, and Halobellus, respectively, based on the comparison of diverse phenotypic characteristics. The polar lipid profiles of these four strains were closely similar to those of respective relatives within the genera Halobacterium, Natronomonas, Halorientalis, and Halobellus, respectively. The phenotypic, phylogenetic, and genome-based analyses indicated that strains YCN1, YCN58, LT38, and LT62 represent respective novel species within the genera Halobacterium, Natronomonas, Halorentalis, and Halobellus, for which the names Halobacterium yunchengense sp. nov., Natronomonas amylolytica sp. nov., Halorientalis halophila sp. nov., and Halobellus salinisoli sp. nov. are proposed, respectively.
四个嗜盐古菌菌株 YCN1、YCN58、LT38 和 LT62 分别从运城盐湖（山西，中国）和塔里木盆地（新疆，中国）中分离得到。系统发育和系统基因组分析表明，这四个菌株分别与嗜盐杆菌、嗜盐碱杆菌、盐生古菌和盐生贝氏菌的相关种紧密聚类。这四个菌株与其相关属种的 AAI、ANI 和 dDDH 值均低于种间划分的建议阈值。基于不同表型特征的比较，菌株 YCN1、YCN58、LT38 和 LT62 可以与嗜盐杆菌、嗜盐碱杆菌、盐生古菌和盐生贝氏菌的现有种区分开来。这四个菌株的极性脂图谱与嗜盐杆菌、嗜盐碱杆菌、盐生古菌和盐生贝氏菌的相应亲缘种非常相似。表型、系统发育和基于基因组的分析表明，菌株 YCN1、YCN58、LT38 和 LT62 分别代表嗜盐杆菌、嗜盐碱杆菌、盐生古菌和盐生贝氏菌属中的四个新种，分别命名为运城嗜盐杆菌 sp. nov.、嗜淀粉盐杆菌 sp. nov.、嗜盐盐生古菌 sp. nov. 和盐生贝氏菌 sp. nov.。
