Division of Neurosurgery, Graduate School of Medicine, Nippon Medical School, Bunkyo-ku, Tokyo, Japan.
Department of Legal Medicine, Graduate School of Medicine, Nippon Medical School, Inzai, Chiba, Japan.
PLoS One. 2024 Mar 8;19(3):e0299641. doi: 10.1371/journal.pone.0299641. eCollection 2024.
Diabetes causes arteriosclerosis, primarily due to persistent hyperglycemia, subsequently leading to various cardiovascular events. No method has been established for directly detecting and evaluating arteriosclerotic lesions from blood samples of diabetic patients, as the mechanism of arteriosclerotic lesion formation, which involves complex molecular biological processes, has not been elucidated. "NMR modal analysis" is a technology that enables visualization of specific nuclear magnetic resonance (NMR) signal properties of blood samples. We hypothesized that this technique could be used to identify changes in blood status associated with the progression of arteriosclerotic lesions in the context of diabetes. The study aimed to assess the possibility of early detection and evaluation of arteriosclerotic lesions by NMR modal analysis of serum samples from diabetes model mice. Diabetes model mice (BKS.Cg db/db) were bred in a clean room and fed a normal diet. Blood samples were collected and centrifuged. Carotid arteries were collected for histological examination by hematoxylin and eosin staining on weeks 10, 14, 18, 22, and 26. The serum was separated and subjected to NMR modal analysis and biochemical examination. Mice typically show hyperglycemia at an early stage (8 weeks old), and pathological findings of a previous study showed that more than half of mice had atheromatous plaques at 18 weeks old, and severe arteriosclerotic lesions were observed in almost all mice after 22 weeks. Partial least squares regression analysis was performed, which showed that the mice were clearly classified into two groups with positive and negative score values within 18 weeks of age. The findings of this study revealed that NMR modal properties of serum are associated with arteriosclerotic lesions. Thus, it may be worth exploring the possibility that the risk of cardiovascular events in diabetic patients could be assessed using serum samples.
糖尿病导致动脉硬化，主要是由于持续的高血糖，随后导致各种心血管事件。目前尚无方法可以直接从糖尿病患者的血液样本中检测和评估动脉硬化病变，因为动脉硬化病变形成的机制涉及复杂的分子生物学过程，尚未阐明。“NMR 模态分析”是一种能够可视化血液样本中特定磁共振（NMR）信号特性的技术。我们假设，这项技术可用于识别与糖尿病患者动脉硬化病变进展相关的血液状态变化。本研究旨在评估通过 NMR 模态分析糖尿病模型小鼠的血清样本，早期检测和评估动脉硬化病变的可能性。在清洁室中繁殖糖尿病模型小鼠（BKS.Cg db/db），并给予正常饮食。采集血液样本并离心。在第 10、14、18、22 和 26 周收集颈动脉进行苏木精和伊红染色的组织学检查。分离血清并进行 NMR 模态分析和生化检查。这些小鼠通常在早期就表现出高血糖（8 周龄），之前的研究病理学结果表明，18 周龄时超过一半的小鼠有动脉粥样斑块，22 周后几乎所有小鼠都出现严重的动脉硬化病变。进行偏最小二乘回归分析，结果表明，在 18 周龄内，这些小鼠可以清楚地分为阳性和阴性评分值两组。本研究结果表明，血清的 NMR 模态特性与动脉硬化病变有关。因此，使用血清样本评估糖尿病患者发生心血管事件的风险，可能值得进一步探索。
