Central European Institute of Technology, Masaryk University, Kamenice 753/5, 625 00, Brno, Czech Republic.
Department of Biology, Faculty of Medicine, Masaryk University, Kamenice 753/5, 625 00, Brno, Czech Republic.
Cell Commun Signal. 2024 Mar 9;22(1):171. doi: 10.1186/s12964-024-01548-3.
This review presents a comprehensive overview of labelling strategies for endogenous and exogenous extracellular vesicles, that can be utilised both in vitro and in vivo. It covers a broad spectrum of approaches, including fluorescent and bioluminescent labelling, and provides an analysis of their applications, strengths, and limitations. Furthermore, this article presents techniques that use radioactive tracers and contrast agents with the ability to track EVs both spatially and temporally. Emphasis is also placed on endogenous labelling mechanisms, represented by Cre-lox and CRISPR-Cas systems, which are powerful and flexible tools for real-time EV monitoring or tracking their fate in target cells. By summarizing the latest developments across these diverse labelling techniques, this review provides researchers with a reference to select the most appropriate labelling method for their EV based research.
本文综述了内源性和外源性细胞外囊泡的标记策略，这些策略可在体外和体内使用。它涵盖了广泛的方法，包括荧光和生物发光标记，并分析了它们的应用、优点和局限性。此外，本文还介绍了使用放射性示踪剂和对比剂的技术，这些技术具有在空间和时间上跟踪 EV 的能力。本文还强调了内源性标记机制，代表了 Cre-lox 和 CRISPR-Cas 系统，它们是实时 EV 监测或跟踪其在靶细胞中命运的强大而灵活的工具。通过总结这些不同标记技术的最新进展，本文为研究人员提供了一个参考，以选择最适合其基于 EV 的研究的标记方法。
