Hata Satoshi, Ihara Shiro, Saito Hikaru, Murayama Mitsuhiro
Faculty of Engineering Sciences, Kyushu University, 6-1 Kasugakoen, Kasuga, Fukuoka 816-8580, Japan.
The Ultramicroscopy Research Center, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
Microscopy (Oxf). 2024 Apr 8;73(2):133-144. doi: 10.1093/jmicro/dfae008.
In-situ observation has expanded the application of transmission electron microscopy (TEM) and has made a significant contribution to materials research and development for energy, biomedical, quantum, etc. Recent technological developments related to in-situ TEM have empowered the incorporation of three-dimensional observation, which was previously considered incompatible. In this review article, we take up heating as the most commonly used external stimulus for in-situ TEM observation and overview recent in-situ TEM studies. Then, we focus on the electron tomography (ET) and in-situ heating combined observation by introducing the authors' recent research as an example. Assuming that in-situ heating observation is expanded from two dimensions to three dimensions using a conventional TEM apparatus and a commercially available in-situ heating specimen holder, the following in-situ heating-and-ET observation procedure is proposed: (i) use a rapid heating-and-cooling function of a micro-electro-mechanical system holder; (ii) heat and cool the specimen intermittently and (iii) acquire a tilt-series dataset when the specimen heating is stopped. This procedure is not too technically challenging and can have a wide range of applications. Essential technical points for a successful 4D (space and time) observation will be discussed through reviewing the authors' example application.
原位观察扩展了透射电子显微镜（TEM）的应用范围，并为能源、生物医学、量子等领域的材料研发做出了重大贡献。与原位TEM相关的最新技术发展使得三维观察得以实现，而三维观察在以前被认为是无法实现的。在这篇综述文章中，我们将加热作为原位TEM观察中最常用的外部刺激因素，并概述近期的原位TEM研究。然后，我们以作者最近的研究为例，重点介绍电子断层扫描（ET）与原位加热相结合的观察方法。假设使用传统的TEM仪器和市售的原位加热样品架将原位加热观察从二维扩展到三维，我们提出了以下原位加热与ET观察程序：（i）使用微机电系统样品架的快速加热和冷却功能；（ii）间歇性地加热和冷却样品；（iii）在样品加热停止时获取倾斜系列数据集。该程序在技术上并非具有太大挑战性，并且具有广泛的应用范围。我们将通过回顾作者的示例应用来讨论成功进行四维（空间和时间）观察的关键技术要点。
