Ginzburg Daniel N, Cox Jack A, Rhee Seung Y
Department of Plant Biology Carnegie Institution for Science Stanford California USA.
Present address: Department of Plant Sciences University of Cambridge Cambridge UK.
Plant Direct. 2024 Mar 7;8(3):e571. doi: 10.1002/pld3.571. eCollection 2024 Mar.
Noninvasive phenotyping can quantify dynamic plant growth processes at higher temporal resolution than destructive phenotyping and can reveal phenomena that would be missed by end-point analysis alone. Additionally, whole-plant phenotyping can identify growth conditions that are optimal for both above- and below-ground tissues. However, noninvasive, whole-plant phenotyping approaches available today are generally expensive, complex, and non-modular. We developed a low-cost and versatile approach to noninvasively measure whole-plant physiology over time by growing plants in isolated hydroponic chambers. We demonstrate the versatility of our approach by measuring whole-plant biomass accumulation, water use, and water use efficiency every two days on unstressed and osmotically stressed sorghum accessions. We identified relationships between root zone acidification and photosynthesis on whole-plant water use efficiency over time. Our system can be implemented using cheap, basic components, requires no specific technical expertise, and should be suitable for any non-aquatic vascular plant species.
非侵入性表型分析能够以比破坏性表型分析更高的时间分辨率量化动态植物生长过程，并且能够揭示仅通过终点分析会遗漏的现象。此外，全株表型分析能够识别对地上和地下组织均为最佳的生长条件。然而，目前可用的非侵入性全株表型分析方法通常昂贵、复杂且非模块化。我们开发了一种低成本且通用的方法，通过在隔离的水培室中种植植物来随时间非侵入性地测量全株生理状况。我们通过每两天对未受胁迫和渗透胁迫的高粱品种测量全株生物量积累、水分利用和水分利用效率，来证明我们方法的通用性。我们确定了根区酸化与光合作用之间随时间对全株水分利用效率的关系。我们的系统可以使用廉价的基本组件来实现，不需要特定的技术专长，并且应该适用于任何非水生维管植物物种。
