Dai Yukai, Xu Qizhong, Xia Manqi, Chen Caimin, Xiong Xinming, Yang Xin, Wang Wei
The Second Clinical College of Guangzhou Medical University, Guangzhou, China.
Department of Thoracic Surgery, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
PeerJ. 2024 Mar 4;12:e17089. doi: 10.7717/peerj.17089. eCollection 2024.
Recent studies have found that circular RNAs (circRNAs) play important roles in tumorigenesis. This study aimed to determine the function and potential mechanisms of hsa_circ_0001615 in esophageal cancer.
Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) was used to validate the expression of hsa_circ_0001615 and miR-142-5p. Subsequently, 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium salt, flow cytometry, clone formation, and transwell assays were used to assess the function of hsa_circ_0001615. Furthermore, qRT-PCR and Western blot analysis were used to verify cyclin D1, Bcl-2 associated X, B-cell lymphoma/leukemia gene-2, and β-catenin levels. Circular RNA Interactome was used to estimate the binding site between hsa_circ_0001615 and miR-142-5p. Additionally, dual-luciferase reporter assays were used to determine whether miR-142-5p was a direct target of hsa_circ_0001615. Pearson correlation analysis was used to explore the relationship between miR-142-5p and hsa_circ_0001615.
In esophageal cancer, the expressions of hsa_circ_0001615 and miR-142-5p were increased and decreased, respectively. Hsa_circ_0001615 inhibition significantly reduced the proliferation, migration, and invasion but increased the apoptosis of esophageal cancer cells. Additionally, hsa_circ_0001615 knockdown increased miR-142-5p expression but decreased β-catenin expression. MiR-142-5p was a direct target of hsa_circ_0001615.
Hsa_circ_0001615 knockdown could mediate antitumor effects through the miR-142-5p/β-catenin pathway.
近期研究发现环状RNA（circRNAs）在肿瘤发生过程中发挥重要作用。本研究旨在确定hsa_circ_0001615在食管癌中的功能及潜在机制。
采用定量实时逆转录聚合酶链反应（qRT-PCR）验证hsa_circ_0001615和miR-142-5p的表达。随后，使用3-(4,5-二甲基噻唑-2-基)-5-(3-羧甲氧基苯基)-2-(4-磺基苯基)-2H-四唑盐、流式细胞术、克隆形成和Transwell实验评估hsa_circ_0001615的功能。此外，采用qRT-PCR和蛋白质免疫印迹分析验证细胞周期蛋白D1、Bcl-2相关X蛋白、B细胞淋巴瘤/白血病基因-2和β-连环蛋白的水平。利用Circular RNA Interactome预测hsa_circ_0001615与miR-142-5p之间的结合位点。另外，使用双荧光素酶报告基因实验确定miR-142-5p是否为hsa_circ_0001615的直接靶点。采用Pearson相关性分析探讨miR-142-5p与hsa_circ_0001615之间的关系。
在食管癌中，hsa_circ_0001615表达升高，而miR-142-5p表达降低。抑制hsa_circ_0001615可显著降低食管癌细胞的增殖、迁移和侵袭能力，但增加其凋亡。此外，敲低hsa_circ_0001615可增加miR-142-5p表达，但降低β-连环蛋白表达。miR-142-5p是hsa_circ_0001615的直接靶点。
敲低hsa_circ_0001615可通过miR-142-5p/β-连环蛋白途径介导抗肿瘤作用。
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