Zheng Yi, Si Yunlong, Xu Xuejiao, Gu Hongming, He Zhen, Zhao Zihan, Feng Zhangkai, Su Jiyong, Mayo Kevin H, Zhou Yifa, Tai Guihua
Engineering Research Center of Glycoconjugates Ministry of Education, Jilin Provincial Key Laboratory of Chemistry and Biology of Changbai Mountain Natural Drugs, School of Life Sciences, Northeast Normal University, Changchun, China.
Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN, USA.
J Ginseng Res. 2024 Mar;48(2):202-210. doi: 10.1016/j.jgr.2023.11.007. Epub 2023 Dec 6.
Meyer polysaccharides exhibit various biological functions, like antagonizing galectin-3-mediated cell adhesion and migration. Galectin-8 (Gal-8), with its linker-joined - and -terminal carbohydrate recognition domains (CRDs), is also crucial to these biological processes, and thus plays a role in various pathological disorders. Yet the effect of ginseng-derived polysaccharides in modulating Gal-8 function has remained unclear.
P. ginseng-derived pectin was chromatographically isolated and enzymatically digested to obtain a series of polysaccharides. Biolayer Interferometry (BLI) quantified their binding affinity to Gal-8, and their inhibitory effects on Gal-8 was assessed by hemagglutination, cell migration and T-cell apoptosis.
Our ginseng-derived pectin polysaccharides consist mostly of rhamnogalacturonan-I (RG-I) and homogalacturonan (HG). BLI shows that Gal-8 binding rests primarily in RG-I and its β-1,4-galactan side chains, with sub-micromolar K values. Both - and -terminal Gal-8 CRDs bind RG-I, with binding correlated with Gal-8-mediated function.
P. ginseng RG-I pectin β-1,4-galactan side chains are crucial to binding Gal-8 and antagonizing its function. This study enhances our understanding of galectin-sugar interactions, information that may be used in the development of pharmaceutical agents targeting Gal-8.
迈耶多糖具有多种生物学功能，如拮抗半乳糖凝集素-3介导的细胞黏附和迁移。半乳糖凝集素-8（Gal-8）具有连接的α-和β-末端碳水化合物识别结构域（CRD），对这些生物学过程也至关重要，因此在各种病理疾病中发挥作用。然而，人参来源的多糖对Gal-8功能的调节作用仍不清楚。
对人参来源的果胶进行色谱分离和酶解，得到一系列多糖。生物层干涉术（BLI）定量了它们与Gal-8的结合亲和力，并通过血凝、细胞迁移和T细胞凋亡评估了它们对Gal-8的抑制作用。
我们从人参中提取的果胶多糖主要由鼠李半乳糖醛酸聚糖-I（RG-I）和同型半乳糖醛酸聚糖（HG）组成。BLI显示，Gal-8的结合主要依赖于RG-I及其β-1,4-半乳聚糖侧链，K值为亚微摩尔级。α-和β-末端Gal-8 CRD均与RG-I结合，且这种结合与Gal-8介导的功能相关。
人参RG-I果胶的β-1,4-半乳聚糖侧链对于结合Gal-8并拮抗其功能至关重要。本研究增进了我们对半乳糖凝集素-糖相互作用的理解，这些信息可用于开发靶向Gal-8的药物。
