Ge An-Hui, Li Qi-Yun, Liu Hong-Wei, Zhang Zheng-Kun, Lu Yang, Liang Zhi-Huai, Singh Brajesh K, Han Li-Li, Xiang Ji-Fang, Xiao Ji-Ling, Liu Si-Yi, Zhang Li-Mei
State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China.
University of Chinese Academy of Sciences, Beijing, China.
Microb Biotechnol. 2024 Mar;17(3):e14435. doi: 10.1111/1751-7915.14435.
The use of microbial inoculant is a promising strategy to improve plant health, but their efficiency often faces challenges due to difficulties in successful microbial colonization in soil environments. To this end, the application of biostimulation products derived from microbes is expected to resolve these barriers via direct interactions with plants or soil pathogens. However, their effectiveness and mechanisms for promoting plant growth and disease resistance remain elusive. In this study, we showed that root irrigation with the extracts of Streptomyces ahygroscopicus strain 769 (S769) solid fermentation products significantly reduced watermelon Fusarium wilt disease incidence by 30% and increased the plant biomass by 150% at a fruiting stage in a continuous cropping field. S769 treatment led to substantial changes in both bacterial and fungal community compositions, and induced a highly interconnected microbial association network in the rhizosphere. The root transcriptome analysis further suggested that S769 treatment significantly improved the expression of the MAPK signalling pathway, plant hormone signal transduction and plant-pathogen interactions, particular those genes related to PR-1 and ethylene, as well as genes associated with auxin production and reception. Together, our study provides mechanistic and empirical evidences for the biostimulation products benefiting plant health through coordinating plant and rhizosphere microbiome interaction.
使用微生物接种剂是改善植物健康的一种有前景的策略，但其效率常常面临挑战，因为在土壤环境中成功进行微生物定殖存在困难。为此，源自微生物的生物刺激产品的应用有望通过与植物或土壤病原体的直接相互作用来解决这些障碍。然而，它们促进植物生长和抗病性的有效性及机制仍不明确。在本研究中，我们发现，在连作田块的结果期，用吸水链霉菌菌株769（S769）固体发酵产物的提取物进行根灌，可使西瓜枯萎病发病率显著降低30%，并使植物生物量增加150%。S769处理导致细菌和真菌群落组成发生显著变化，并在根际诱导形成了一个高度相互连接的微生物关联网络。根系转录组分析进一步表明，S769处理显著提高了丝裂原活化蛋白激酶（MAPK）信号通路、植物激素信号转导和植物 - 病原体相互作用相关基因的表达，特别是那些与病程相关蛋白1（PR - 1）和乙烯相关的基因，以及与生长素产生和接收相关的基因。总之，我们的研究为生物刺激产品通过协调植物与根际微生物组相互作用来促进植物健康提供了机制和实证依据。
