Department of Molecular and Cellular Biology, The Scripps Research Institute; Department of Molecular Medicine, The Scripps Research Institute; Department of Neuroscience, The Scripps Research Institute.
Department of Molecular and Cellular Biology, The Scripps Research Institute; Department of Molecular Medicine, The Scripps Research Institute; Department of Neuroscience, The Scripps Research Institute;
J Vis Exp. 2024 Feb 23(204). doi: 10.3791/66422.
Feeding is an essential biological process for an organism's growth, reproduction, and survival. This assay aims to measure the food intake of Caenorhabditis elegans (C. elegans), an important parameter when studying the genetics of aging or metabolism. In most species, feeding is determined by measuring the difference between the amount of food provided and the amount left after a given time interval. The method presented here uses the same strategy to determine the feeding of C. elegans. It measures the amount of bacteria, the food source of C. elegans, cleared within 72 h. This method uses 96-well microtiter plates and has allowed the screening of hundreds of drugs for their ability to modulate food intake at a speed and depth not possible in other animal models. The strength of this assay is that it allows to measure feeding and lifespan simultaneously and directly measures the disappearance of food and, thus, is based on the same principles used for other organisms, facilitating species-to-species comparison.
摄食是生物体生长、繁殖和生存的基本生物过程。本实验旨在测量秀丽隐杆线虫（C. elegans）的食物摄入量，这是研究衰老或代谢遗传学的重要参数。在大多数物种中，通过测量在给定时间间隔内提供的食物量与剩余的食物量之间的差异来确定摄食量。本文介绍的方法使用相同的策略来确定 C. elegans 的摄食情况。它测量在 72 小时内清除的细菌量，即 C. elegans 的食物源。该方法使用 96 孔微量滴定板，已允许筛选数百种药物，以研究它们在调节摄食方面的能力，这种速度和深度在其他动物模型中是不可能实现的。该实验的优势在于它可以同时测量摄食和寿命，并直接测量食物的消失，因此基于与其他生物体相同的原理，便于物种间的比较。
