Chen Lihua, Dong Binhua, Gao Hangjing, Xue Huifeng, Pan Diling, Sun Pengming
Department of Gynecology, Fujian Provincial Maternity and Child Hospital, Affiliated Hospital of Fujian Medical University, Fuzhou, People's Republic of China.
Fujian Provincial Key Laboratory of Women and Children Major Disease, Fujian Provincial Maternity and Child Hospital, Affiliated Hospital of Fujian Medical University, Fuzhou, People's Republic of China.
J Inflamm Res. 2020 Oct 29;13:813-821. doi: 10.2147/JIR.S278911. eCollection 2020.
Human papillomavirus-16 (HPV-16) is the most carcinogenic HPV genotype. This study aimed to evaluate the clinical value of POU5F1B and HPV-16-E2/E6 by cervical cytology specimens to predict the cervical intraepithelial neoplasia two grade and more (CIN2+).
Finally, 248 patients with HPV-16 single infection were enrolled. Using cytology specimen by real-time quantitative PCR (qPCR), POU5F1B mRNA and HPV-16-E2/E6 were detected. The relationship of POU5F1B, HPV-16-E2/E6 and CIN2+ were analyzed, and the optimal cut-off values of POU5F1B and HPV-16-E2/E6 to predict CIN2+ were calculated.
The mean HPV-16-E2/E6 decreased significantly with cervical lesions development, especially compared with CIN2+ (p<0.05). And the POU5F1B demonstrated higher expression in CIN2+ than that of normal cervical tissue and CIN1 (p<0.05). What is more, POU5F1B was negatively correlated with HPV-16-E2/E6. It demonstrated that the area under the receiver operating characteristic curve (AUC) for POU5F1B (0.9058) was higher than that for HPV-16-E2/E6 (0.8983), and the sensitivity and specificity of POU5F1B in the diagnosis of CIN2+ were higher than HPV-E2/E6. Furthermore, it demonstrated that the POU5F1B had the highest odds ratio (OR= 16.84; 95% CI (8.00-35.46)) for the detection of CIN 2+.
HPV-16-E2/E6≤0.6471 or POU5F1B≥1.0310 in cervical exfoliated cells can be used as a reliable predictor of CIN2+. POU5F1B can be used as a new auxiliary biomarker to determine the HPV infection status and a reliable predictor of CIN2+. The expression of POU5F1B≥1.0310 had the highest OR for the detection of CIN2+.
人乳头瘤病毒16型（HPV - 16）是最具致癌性的HPV基因型。本研究旨在通过宫颈细胞学标本评估POU5F1B和HPV - 16 - E2/E6预测宫颈上皮内瘤变二级及以上（CIN2 +）的临床价值。
最终纳入248例HPV - 16单感染患者。采用实时定量聚合酶链反应（qPCR）检测细胞学标本中的POU5F1B mRNA和HPV - 16 - E2/E6。分析POU5F1B、HPV - 16 - E2/E6与CIN2 +的关系，并计算预测CIN2 +的POU5F1B和HPV - 16 - E2/E6的最佳截断值。
随着宫颈病变发展，HPV - 16 - E2/E6均值显著降低，尤其是与CIN2 +相比（p<0.05）。POU5F1B在CIN2 +中的表达高于正常宫颈组织和CIN1（p<0.05）。此外，POU5F1B与HPV - 16 - E2/E6呈负相关。结果显示，POU5F1B的受试者工作特征曲线下面积（AUC）（0.9058）高于HPV - 16 - E2/E6（0.8983），POU5F1B诊断CIN2 +的敏感性和特异性高于HPV - E2/E6。此外，结果表明POU5F1B检测CIN 2 +的比值比最高（OR = 16.84；95% CI（8.00 - 35.46））。
宫颈脱落细胞中HPV - 16 - E2/E6≤0.6471或POU5F1B≥1.0310可作为CIN2 +的可靠预测指标。POU5F1B可作为确定HPV感染状态的新型辅助生物标志物及CIN2 +的可靠预测指标。POU5F1B≥1.0310的表达检测CIN2 +的OR最高。
