Hussein Shaimaa, Ben Bacha Abir, Alonazi Mona, Alwaili Maha Abdullah, Mobasher Maysa A, Alburae Najla Ali, Banjabi Abeer A, El-Said Karim Samy
Department of Pharmacology, College of Pharmacy, Jouf University, Sakaka, Al Jawf, Saudi Arabia.
Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.
Front Mol Biosci. 2024 Feb 26;11:1365440. doi: 10.3389/fmolb.2024.1365440. eCollection 2024.
Cadmium (Cd) is a harmful heavy metal that results in many toxic issues. showed potential pharmaceutical applications. This study investigated the possible ameliorative mechanism of leaves extract (UPLE) against hepatotoxicity induced by cadmium chloride (CdCl) in mice. phytochemical screening and the metal-chelating activity of UPLE were ascertained. Four groups of forty male mice were used ( = 10) as follows; Group 1 (G1) was a negative control. G2 was injected i.p., with UPLE (100 mg/kg b. wt) daily. G3 was injected i.p., with Cd (5 mg/kg b. wt) daily. G4 was injected with Cd as in G3 and with UPLE as in G2. On day 11, the body weight changes were evaluated, blood, and serum samples were collected for hematological and biochemical assessments. Liver tissues were used for biochemical, molecular, and histopathological investigations. The results showed that UPLE contains promising secondary metabolites that considerably lessen the negative effects of Cd on liver. Furthermore, UPLE inhibited oxidative stress and inflammation; restored antioxidant molecules; and promoted nuclear-related factor-2 (Nrf-2) expression. Also, UPLE improved the histopathological alterations induced by Cd. This study explored the beneficial role of UPLE treatment in Cd-induced liver injury through enhancing Nrf-2 signaling and antioxidant enzyme gene expression in the liver of mice. Therefore, UPLE could have valuable implications against hepatotoxicity induced by environmental cadmium exposure. Which can be used as a chelating agent against Cd.
镉（Cd）是一种有害重金属，会引发许多毒性问题，显示出潜在的药用价值。本研究调查了乌羽玉叶提取物（UPLE）对小鼠氯化镉（CdCl）诱导的肝毒性的可能改善机制，确定了UPLE的植物化学筛选和金属螯合活性。使用四组，每组10只雄性小鼠，具体如下：第1组（G1）为阴性对照。G2组每天腹腔注射UPLE（100毫克/千克体重）。G3组每天腹腔注射镉（5毫克/千克体重）。G4组按G3组注射镉，按G2组注射UPLE。在第11天，评估体重变化，采集血液和血清样本进行血液学和生化评估。肝脏组织用于生化、分子和组织病理学研究。结果表明，UPLE含有有前景的次生代谢产物，可显著减轻镉对肝脏的负面影响。此外，UPLE抑制氧化应激和炎症；恢复抗氧化分子；促进核相关因子-2（Nrf-2）表达。同时，UPLE改善了镉诱导的组织病理学改变。本研究通过增强小鼠肝脏中Nrf-2信号传导和抗氧化酶基因表达，探讨了UPLE治疗对镉诱导的肝损伤的有益作用。因此，UPLE对环境镉暴露诱导的肝毒性可能具有重要意义，可作为一种抗镉螯合剂。
