Gao Feng, Liang Wenyu, Chen Qixin, Chen Bairu, Liu Yuchen, Liu Zhibo, Xu Xu, Zhu Rongrong, Cheng Liming
Department of Orthopedics, Tongji Hospital Affiliated to Tongji University, School of Medicine, Tongji University, Shanghai 200331, China.
Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of Ministry of Education, School of Life Science and Technology, Tongji University, Shanghai 200065, China.
Nanomaterials (Basel). 2024 Feb 20;14(5):389. doi: 10.3390/nano14050389.
Disordered reactive oxygen/nitrogen species are a common occurrence in various diseases, which usually cause cellular oxidative damage and inflammation. Despite the wide range of applications for biomimetic nanoparticles with antioxidant or anti-inflammatory properties, designs that seamlessly integrate these two abilities with a synergistic effect in a simple manner are seldom reported. In this study, we developed a novel PEI-Mn composite nanoparticle (PM NP) using a chelation method, and the curcumin was loaded onto PM NPs via metal-phenol coordination to form PEI-Mn@curcumin nanoparticles (PMC NPs). PMC NPs possessed excellent dispersibility and cytocompatibility, was engineered to serve as an effective nanozyme, and exhibited specific SOD-like and CAT-like activities. In addition, the incorporation of curcumin granted PMC NPs the ability to effectively suppress the expression of inflammatory cytokines in microglia induced by LPS. As curcumin also has antioxidant properties, it further amplified the synergistic efficiency of ROS scavenging. Significantly, PMC NPs effectively scavenged ROS triggered by HO in SIM-A9 microglia cells and Neuro-2a cells. PMC NPs also considerably mitigated DNA and lipid oxidation in Neuro-2a cells and demonstrated an increase in cell viability under various HO concentrations. These properties suggest that PMC NPs have significant potential in addressing excessive ROS and inflammation related to neural diseases.
活性氧/氮物种紊乱在各种疾病中普遍存在，通常会导致细胞氧化损伤和炎症。尽管具有抗氧化或抗炎特性的仿生纳米颗粒有广泛应用，但很少有设计能以简单的方式将这两种能力无缝整合并产生协同效应的报道。在本研究中，我们采用螯合方法制备了一种新型的聚乙二醇化锰复合纳米颗粒（PM NP），并通过金属-酚配位将姜黄素负载到PM NPs上，形成聚乙二醇化锰@姜黄素纳米颗粒（PMC NPs）。PMC NPs具有优异的分散性和细胞相容性，被设计用作一种有效的纳米酶，并表现出特定的超氧化物歧化酶样和过氧化氢酶样活性。此外，姜黄素的掺入赋予了PMC NPs有效抑制脂多糖诱导的小胶质细胞中炎性细胞因子表达的能力。由于姜黄素也具有抗氧化特性，它进一步增强了活性氧清除的协同效率。值得注意的是，PMC NPs有效地清除了SIM-A9小胶质细胞和Neuro-2a细胞中由羟基自由基引发的活性氧。PMC NPs还显著减轻了Neuro-2a细胞中的DNA和脂质氧化，并在各种羟基自由基浓度下显示出细胞活力的增加。这些特性表明，PMC NPs在解决与神经疾病相关的过量活性氧和炎症方面具有巨大潜力。
