评估银纳米颗粒和抗菌组合对手术缝线的抗菌活性和生物膜预防作用。
Assessing silver nanoparticle and antimicrobial combinations for antibacterial activity and biofilm prevention on surgical sutures.
机构信息
Department of Pharmaceutics and Pharmaceutical Technology, School of Pharmacy, University of Jordan, Amman 11942, Jordan.
Hamdi Mango Center for Scientific Research, The University of Jordan, Amman 11942, Jordan.
出版信息
J Appl Microbiol. 2024 Apr 1;135(4). doi: 10.1093/jambio/lxae063.
AIMS
To evaluate the effect of silver nanoparticles alone and in combination with Triclosan, and trans-cinnamaldehyde against Staphylococcus aureus and Escherichia coli biofilms on sutures to improve patients' outcomes.
METHODS AND RESULTS
Silver nanoparticles were prepared by chemical method and characterized by UV-visible spectrophotometer and dynamic light scattering. The minimum inhibitory concentration was assessed by the Microdilution assay. The antibiofilm activity was determined using crystal violet assay. A checkerboard assay using the fractional inhibitory concentration index and time-kill curve was used to investigate the synergistic effect of silver nanoparticle combinations. The hemolytic activity was determined using an erythrocyte hemolytic assay. Our results revealed that silver nanoparticles, Triclosan, and trans-cinnamaldehyde (TCA) inhibited S.aureus and E.coli biofilms. Silver nanoparticles with TCA showed a synergistic effect (FICI values 0.35 and 0.45 against S. aureus and E. coli biofilms, respectively), and silver nanoparticles with Triclosan showed complete inhibition of S. aureus biofilm. The hemolytic activity was <2.50% for the combinations.
目的
评估单独使用和联合使用载银纳米粒子与三氯生和肉桂醛(trans-cinnamaldehyde)对抗金黄色葡萄球菌和大肠杆菌缝线生物膜的效果，以改善患者的预后。
方法和结果
采用化学方法制备载银纳米粒子，并通过紫外-可见分光光度计和动态光散射进行表征。采用微量稀释法评估最小抑菌浓度。采用结晶紫法测定抗生物膜活性。使用棋盘微量稀释法和时间杀伤曲线来研究载银纳米粒子组合的协同作用。采用红细胞溶血试验测定溶血活性。我们的结果表明，载银纳米粒子、三氯生和肉桂醛（TCA）抑制了金黄色葡萄球菌和大肠杆菌的生物膜。载银纳米粒子与 TCA 显示出协同作用（对金黄色葡萄球菌和大肠杆菌生物膜的 FICI 值分别为 0.35 和 0.45），而载银纳米粒子与三氯生则完全抑制了金黄色葡萄球菌生物膜的形成。这些组合的溶血活性均<2.50%。
Zotero 插件