do Vale Pereira Gabriella, Teixeira Carla, Couto José, Dias Jorge, Rema Paulo, Gonçalves Ana Teresa
SPAROS Lda., 8700-221 Olhão, Portugal.
Aquaculture Department, Center of Agrarian Sciences, Federal University of Santa Catarina (UFSC), Florianópolis 88061-600, Brazil.
Animals (Basel). 2024 Feb 24;14(5):714. doi: 10.3390/ani14050714.
Dietary protein quality plays a key role in maintaining intestinal mucosal integrity, but also modulates the growth of luminal microorganisms. This work assessed the effect of dietary protein sources on the performance, gut morphology, and microbiome in Nile tilapia. Four isonitrogenous and isolipidic diets comprising equivalent amounts of the protein supply derived from either PLANT, ANIMAL, INSECT, or BACTERIAL (bacterial biomass) sources were fed to triplicate groups of fish (IBW: 12 g) for 46 days. Fish fed the ANIMAL and BACTERIAL diets showed significantly higher weight gains than those fed the PLANT and INSECT diets ( < 0.05). Relative abundance at the phylum level showed that Bacteroidetes, Fusobacteria, and Proteobacteria were the more abundant phyla in tilapia's intestine, while was the most representative genus in all treatments. Interesting patterns were observed in the correlation between amino acid intake and genus and species abundance. Metabolism prediction analysis showed that BACTERIAL amine and polyamine degradation pathways are modulated depending on diets. In conclusion, different protein sources modulate the relationship between bacteria functional pathways and amino acid intake.
膳食蛋白质质量在维持肠道黏膜完整性方面起着关键作用，同时也调节肠腔微生物的生长。这项研究评估了膳食蛋白质来源对尼罗罗非鱼生长性能、肠道形态和微生物群的影响。将四种等氮、等脂的饲料投喂给三组重复的尼罗罗非鱼（初始体重：12克），这四种饲料所含蛋白质分别来源于植物、动物、昆虫或细菌（细菌生物量），且蛋白质供应量相等，投喂期为46天。结果显示，摄食动物源和细菌源饲料的尼罗罗非鱼的体重增幅显著高于摄食植物源和昆虫源饲料的尼罗罗非鱼（P<0.05）。在门水平上的相对丰度显示，拟杆菌门、梭杆菌门和变形菌门是尼罗罗非鱼肠道中丰度较高的门类，而在所有处理组中，[此处原文缺失具体属名]是最具代表性的属。在氨基酸摄入量与属和种的丰度之间观察到有趣的相关性模式。代谢预测分析表明，细菌胺和多胺降解途径会因饲料不同而受到调节。总之，不同的蛋白质来源会调节细菌功能途径与氨基酸摄入量之间的关系。
