S100蛋白作为口腔病变恶性转化风险评估中的生物标志物

S100 Proteins as Biomarkers in Risk Estimations for Malignant Transformation in Oral Lesions.

作者信息

Probstmeier Rainer, Kraus Dominik, Wenghoefer Matthias, Winter Jochen

机构信息

Neuro- and Tumor Cell Biology Group, Department of Nuclear Medicine, University Hospital Bonn, University of Bonn, Bonn, Germany.

Department of Prosthodontics, Preclinical Education, and Material Sciences, University of Bonn, Bonn, Germany.

出版信息

Methods Mol Biol. 2019;1929:763-771. doi: 10.1007/978-1-4939-9030-6_48.

DOI:10.1007/978-1-4939-9030-6_48

PMID:30710310

Abstract

Oncologic relevant members of S100 proteins are described as promising biomarkers in molecular pathology for risk estimation in oral neoplasia exhibiting different stages of malignancy: gingiva as healthy tissue, irritation fibroma as benign, leukoplakia as precancerous, and oral squamous cell carcinoma as malignant entity. Gene expression levels of S100A4 (metastasin), S100A7 (psoriasin), S100A8 (calgranulin A), and S100A9 (calgranulin B) were analyzed using quantitative RT-PCR. In addition, immunohistochemistry-based microscopy was used to examine cellular localization and distribution of these biomarkers in tissue sections. The results indicate that S100 proteins represent promising biomarkers for early-stage diagnosis in oral lesions. The inclusion of expression profiles and ratios for each entity even improves their diagnostic validity.

摘要

S100蛋白的肿瘤相关成员被描述为分子病理学中很有前景的生物标志物，可用于评估口腔肿瘤不同恶性阶段的风险，这些阶段包括：作为健康组织的牙龈、作为良性病变的刺激性纤维瘤、作为癌前病变的白斑以及作为恶性实体的口腔鳞状细胞癌。使用定量逆转录聚合酶链反应（qRT-PCR）分析了S100A4（转移素）、S100A7（牛皮癣素）、S100A8（钙粒蛋白A）和S100A9（钙粒蛋白B）的基因表达水平。此外，基于免疫组织化学的显微镜检查用于检测这些生物标志物在组织切片中的细胞定位和分布。结果表明，S100蛋白是口腔病变早期诊断很有前景的生物标志物。纳入每个实体的表达谱和比率甚至提高了它们的诊断有效性。