Jiang Yu-Na, Tan Manqi, He Chenglong, Wang Jiaxi, Wei Yi, Jing Ningning, Wang Bing, Yang Fang, Zhang Yujie, Li Meng
Cixi Biomedical Research Institute, Wenzhou Medical University, Ningbo 315302, China.
Ningbo Key Laboratory of Biomedical Imaging Probe Materials and Technology, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, China.
Molecules. 2024 Feb 28;29(5):1040. doi: 10.3390/molecules29051040.
The global antibiotic resistance crisis has drawn attention to the development of treatment methods less prone to inducing drug resistance, such as antimicrobial photodynamic therapy (aPDT). However, there is an increasing demand for new photosensitizers capable of efficiently absorbing in the near-infrared (NIR) region, enabling antibacterial treatment in deeper sites. Additionally, advanced strategies need to be developed to avert drug resistance stemming from prolonged exposure. Herein, we have designed a conjugated oligoelectrolyte, namely TTQAd, with a donor-acceptor-donor (D-A-D) backbone, enabling the generation of reactive oxygen species (ROS) under NIR light irradiation, and cationic adamantaneammonium groups on the side chains, enabling the host-guest interaction with curcubit[7]uril (CB7). Due to the amphiphilic nature of TTQAd, it could spontaneously form nanoassemblies in aqueous solution. Upon CB7 treatment, the positive charge of the cationic adamantaneammonium group was largely shielded by CB7, leading to a further aggregation of the nanoassemblies and a reduced antibacterial efficacy of TTQAd. Subsequent treatment with competitor guests enables the release of TTQAd and restores its antibacterial effect. The reversible supramolecular switch for regulating the antibacterial effect offers the potential for the controlled release of active photosensitizers, thereby showing promise in preventing the emergence of drug-resistant bacteria.
全球抗生素耐药性危机已引起人们对开发不易诱导耐药性的治疗方法的关注，例如抗菌光动力疗法（aPDT）。然而，对能够在近红外（NIR）区域有效吸收的新型光敏剂的需求日益增加，这使得在更深部位进行抗菌治疗成为可能。此外，需要开发先进的策略来避免因长期暴露而产生的耐药性。在此，我们设计了一种共轭寡电解质，即TTQAd，其具有供体-受体-供体（D-A-D）主链，能够在近红外光照射下产生活性氧（ROS），并且在侧链上带有阳离子金刚烷铵基团，能够与葫芦[7]脲（CB7）发生主客体相互作用。由于TTQAd的两亲性，它可以在水溶液中自发形成纳米聚集体。经CB7处理后，阳离子金刚烷铵基团的正电荷被CB7大量屏蔽，导致纳米聚集体进一步聚集，TTQAd的抗菌效果降低。随后用竞争客体进行处理能够使TTQAd释放并恢复其抗菌效果。用于调节抗菌效果的可逆超分子开关为活性光敏剂的控释提供了潜力，从而在防止耐药菌出现方面显示出前景。
