Hunan Provincial Key Laboratory for Synthetic Biology of Traditional Chinese Medicine, School of Pharmaceutical Sciences, Hunan University of Medicine, Huaihua, Hunan, China.
PeerJ. 2023 Apr 17;11:e15212. doi: 10.7717/peerj.15212. eCollection 2023.
The auxin/indole-3-acetic acid (Aux/IAA) gene family serves as a principal group of genes responsible for modulating plant growth and development through the auxin signaling pathway. Despite the significance of this gene family, the identification and characterization of members within the well-known Chinese medicinal herb () have not been thoroughly investigated. In this study, we employed bioinformatics methods to identify 23 Aux/IAA genes within the genome of . These genes were classified into typical IAA and atypical IAA based on their domain structure. Our analysis of the promoter regions revealed that the expression of these genes is regulated not only by auxins, but also by other hormones and environmental factors. Furthermore, we found that the expression patterns of these genes varied across various tissues of . While our initial hypothesis suggested that the primary function of these genes was the interaction between and , gene co-expression network analysis revealed that they are also influenced by various other transcription factors, such as and . The findings establish a sturdy basis for future investigations into the function of the Aux/IAA gene family and exhibit promising prospects for enhancing the genetics of this medicinal flora and its associated species.
生长素/吲哚-3-乙酸（Aux/IAA）基因家族是通过生长素信号通路调节植物生长和发育的主要基因家族之一。尽管这个基因家族意义重大，但在中国著名的药用植物 （）中，其成员的鉴定和特征研究尚未得到彻底调查。在这项研究中，我们采用生物信息学方法在 的基因组中鉴定了 23 个 Aux/IAA 基因。这些基因根据其结构域结构分为典型 IAA 和非典型 IAA。我们对启动子区域的分析表明，这些基因的表达不仅受到生长素的调控，还受到其他激素和环境因素的调控。此外，我们发现这些基因在 的不同组织中的表达模式存在差异。虽然我们的初步假设是这些基因的主要功能是 和 之间的相互作用，但基因共表达网络分析表明，它们也受到其他转录因子的影响，如 和 。这些发现为进一步研究 Aux/IAA 基因家族的功能奠定了坚实的基础，并为增强这种药用植物及其相关物种的遗传学提供了广阔的前景。
