CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, 650223, China.
College of Life Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China.
BMC Microbiol. 2022 Mar 9;22(1):70. doi: 10.1186/s12866-022-02486-1.
The plant microbiome is vital for plant health, fitness, and productivity. Interestingly, plant metabolites and the plant microbiome can influence each other. The combination of metabolomics and microbiome may reveal the critical links between the plant and its microbiome. It is of great significance to agricultural production and human health, especially for Chinese medicine research. Aconitum vilmorinianum Kom. is a herb with alkaloid activities, and its roots are the raw material for some Chinese medicines. Former studies have investigated alkaloidal metabolites and antibacterial activities of endophytes in A. vilmorinianum roots. However, there are limited reports on the root microbiota that can influence the alkaloidal metabolome of A. vilmorinianum.
This research used ultra performance liquid chromatography-tandem mass spectrometry technology and high-throughput sequencing to examine the alkaloidal metabolome, bacterial microbiota, and fungal microbiota in A. vilmorinianum roots at two different sites in China. The results revealed that the samples from the two sites were rich in distinct alkaloidal metabolites and recruited significantly different root microbiota. Based on bioinformatics analysis, we found the potential bacterial and fungal microbiota impacting the alkaloidal metabolome in A. vilmorinianum.
Our findings reveal the composition of the alkaloidal metabolome, bacterial root microbiota, and fungal root microbiota in A. vilmorinianum roots at two different sites. Potential root microbiota that can influence the alkaloidal metabolome of A. vilmorinianum are indicated. This study provides a strategy for the cultivation and research of A. vilmorinianum and other Chinese herbs.
植物微生物组对植物的健康、适应性和生产力至关重要。有趣的是，植物代谢物和植物微生物组可以相互影响。代谢组学和微生物组学的结合可能揭示植物与其微生物组之间的关键联系。这对于农业生产和人类健康具有重要意义，特别是对于中药研究。乌头属植物是一种具有生物碱活性的草本植物，其根是一些中药的原料。以前的研究已经调查了乌头属植物根内的内生菌的生物碱代谢物和抗菌活性。然而，关于影响乌头属植物生物碱代谢组的根微生物组的报道有限。
本研究采用超高效液相色谱-串联质谱技术和高通量测序技术，研究了中国两个不同地点的乌头属植物根中的生物碱代谢组、细菌微生物群和真菌微生物群。结果表明，两个地点的样本富含独特的生物碱代谢物，并招募了明显不同的根微生物群。基于生物信息学分析，我们发现了潜在的细菌和真菌微生物群，它们可能影响乌头属植物的生物碱代谢组。
本研究揭示了两个不同地点的乌头属植物根中的生物碱代谢组、细菌根微生物群和真菌根微生物群的组成。指出了可能影响乌头属植物生物碱代谢组的潜在根微生物群。本研究为乌头属植物和其他中药的栽培和研究提供了一种策略。
