Department of Experimental Medicine, Histology and Embryology Section, University of Campania "L. Vanvitelli", Naples, Italy.
Methods Mol Biol. 2024;2777:35-49. doi: 10.1007/978-1-0716-3730-2_3.
Over the past 20 years, there has been a lot of interest in the study and investigation of cancer stem cells (CSCs) or tumor-initiating cells (TICs). CSCs are rare, dormant cells and able to self-renew and maintain tumor development and heterogeneity. A new age of basic and clinical cancer research, reclassification of human tumors, and the development of novel therapeutic approaches will undoubtedly result from a better knowledge of CSCs. In order to develop effective and therapeutic strategies to treat cancer, it is crucial to understand the basic characteristics of CSCs, their importance to cancer therapy, and methodologies to isolate, detect, and characterize them. Here, we outline the main methods and protocols to identify, isolate, and culture CSCs from primary tumors.
在过去的 20 年中，人们对癌症干细胞（CSC）或肿瘤起始细胞（TIC）的研究和调查产生了浓厚的兴趣。CSC 是罕见的休眠细胞，能够自我更新并维持肿瘤的发展和异质性。对 CSC 的更好认识将无疑带来基础和临床癌症研究的新时代、人类肿瘤的重新分类以及新型治疗方法的发展。为了开发治疗癌症的有效治疗策略，了解 CSC 的基本特征、它们对癌症治疗的重要性以及分离、检测和鉴定它们的方法学至关重要。在这里，我们概述了从原发性肿瘤中识别、分离和培养 CSC 的主要方法和方案。
