Department of Experimental Medicine, Histology and Embryology Section, University of Campania "L. Vanvitelli", Naples, Italy.
Methods Mol Biol. 2024;2835:215-228. doi: 10.1007/978-1-0716-3995-5_18.
Cancer stem cells (CSCs) have emerged as an attractive research interest due to their prominent role in development of the tumors. CSCs are rare dormant cells that can self-renew and maintain tumor development and heterogeneity. A better understanding of CSCs can improve tumor classification and contribute toward the development of novel therapeutic approaches to fight cancer. Hence, it is of immense importance to comprehend the basic function of CSCs in tumor formation, which can only be possible by devising perfected methodologies to isolate, detect, and characterize them. In this chapter, we outline the key protocols to culture, identify, and isolate CSCs from solid tumors to further advance basic and clinical investigation related to CSCs and their role in tumor biology.
癌症干细胞（CSC）因其在肿瘤发生中的突出作用而成为一个备受关注的研究领域。CSC 是罕见的休眠细胞，能够自我更新并维持肿瘤的发生和异质性。更好地了解 CSC 可以改善肿瘤分类，并有助于开发新的治疗方法来对抗癌症。因此，理解 CSC 在肿瘤形成中的基本功能非常重要，而这只有通过设计完善的方法来分离、检测和表征 CSC 才有可能。在本章中，我们概述了从实体瘤中培养、鉴定和分离 CSC 的关键方案，以进一步推进与 CSC 及其在肿瘤生物学中的作用相关的基础和临床研究。
