Chae Jayoung, Choi Yonghyun, Hong Joohye, Kim Namju, Kim Jiwon, Lee Hee-Young, Choi Jonghoon
School of Integrative Engineering, Chung-Ang University, Seoul 06974, Republic of Korea.
Feynman Institute of Technology, Nanomedicine Corporation, Seoul 06974, Republic of Korea.
ACS Appl Bio Mater. 2024 Apr 15;7(4):2175-2185. doi: 10.1021/acsabm.3c01145. Epub 2024 Mar 13.
Lung cancer and complex infection are lung diseases associated with high incidence and mortality rates. Most conventional anticancer drugs and antibiotics have certain limitations, including high drug resistance rates and adverse effects. Herein, we aimed to synthesize mannose surface-modified solid lipid nanoparticles (SLNs) loaded with curcumin (Man-CUR SLN) for the effective treatment of lung disease. The synthesized Man-CUR SLNs were analyzed using various instrumental techniques for structural and physicochemical characterization. Loading curcumin into SLNs improved the encapsulation efficiency and drug release capacity, as demonstrated by high-performance liquid chromatography analysis. Furthermore, we characterized the anticancer effect of curcumin using the A549 lung cancer cell line. Cells treated with Man-CUR SLN exhibited an increased cellular uptake and cytotoxicity. Moreover, treatment with free CUR could more effectively reduce cancer migration than treatment with Man-CUR SLNs. Similarly, free curcumin elicited a stronger apoptosis-inducing effect than that of Man-CUR SLNs, as demonstrated by reverse transcription-quantitative PCR analysis. Finally, we examined the antibacterial effects of free curcumin and Man-CUR SLNs against () and infected macrophages, revealing that Man-CUR SLNs exerted the strongest antibacterial effect. Collectively, these findings indicate that mannose-receptor-targeted curcumin delivery using lipid nanoparticles could be effective in treating lung diseases. Accordingly, this drug delivery system can be used to target a variety of cancers and immune cells.
肺癌和复杂感染是发病率和死亡率都很高的肺部疾病。大多数传统抗癌药物和抗生素都有一定的局限性，包括高耐药率和副作用。在此，我们旨在合成负载姜黄素的甘露糖表面修饰固体脂质纳米粒（Man-CUR SLN），用于有效治疗肺部疾病。使用各种仪器技术对合成的Man-CUR SLN进行分析，以进行结构和理化表征。高效液相色谱分析表明，将姜黄素负载到SLN中提高了包封效率和药物释放能力。此外，我们使用A549肺癌细胞系表征了姜黄素的抗癌作用。用Man-CUR SLN处理的细胞表现出细胞摄取增加和细胞毒性增强。此外，与用Man-CUR SLN处理相比，用游离姜黄素处理能更有效地减少癌细胞迁移。同样，如逆转录定量PCR分析所示，游离姜黄素比Man-CUR SLN诱导凋亡的作用更强。最后，我们检测了游离姜黄素和Man-CUR SLN对（）和感染巨噬细胞的抗菌作用，发现Man-CUR SLN具有最强的抗菌效果。总的来说，这些发现表明，使用脂质纳米粒靶向甘露糖受体递送姜黄素可能对治疗肺部疾病有效。因此，这种药物递送系统可用于靶向多种癌症和免疫细胞。
