Department of Biology, Kish International Campus, University of Tehran, Tehran, Iran.
Department of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.
Reprod Sci. 2024 Jul;31(7):2114-2122. doi: 10.1007/s43032-024-01507-w. Epub 2024 Mar 13.
This study investigated the deleterious impact of advanced glycation end products (AGEs), commonly present in metabolic disorders like diabetes, obesity, and infertility-related conditions, on sperm structure and function using a mouse model where AGE generation was heightened through dietary intervention. Five-week-old C57BL/6 mice were divided into two groups, one on a regular diet (control) and the other on an AGE-rich diet. After 13 weeks, various parameters were examined, including fasting blood glucose, body weight, food consumption, sperm parameters and function, testicular superoxide dismutase levels, malondialdehyde content, total antioxidant capacity, Johnson score, AGE receptor (RAGE) content, and carboxymethyl lysine (CML) content. The results showed that mice in the AGE group exhibited increased body weight and elevated fasting blood glucose levels. Furthermore, the AGE group displayed adverse effects on sperm, including reduced sperm counts, motility, increased morphological abnormalities, residual histone, protamine deficiency, sperm DNA fragmentation, reduced testicular antioxidant capacity, and higher levels of RAGE and CML proteins. These findings underscore the negative impact of AGEs on male reproductive health, particularly within the context of metabolic disorders, emphasizing the crucial role of the AGE/RAGE axis in male infertility, especially in the context of Western dietary patterns.
本研究使用一种通过饮食干预来提高晚期糖基化终产物（AGE）生成的小鼠模型，探讨了代谢紊乱（如糖尿病、肥胖和与不孕相关的病症）中常见的 AGE 对精子结构和功能的有害影响。将 5 周龄 C57BL/6 小鼠分为两组，一组给予常规饮食（对照组），另一组给予富含 AGE 的饮食。13 周后，检查了各种参数，包括空腹血糖、体重、食物消耗、精子参数和功能、睾丸超氧化物歧化酶水平、丙二醛含量、总抗氧化能力、约翰逊评分、AGE 受体（RAGE）含量和羧甲基赖氨酸（CML）含量。结果表明，AGE 组小鼠体重增加，空腹血糖水平升高。此外，AGE 组对精子产生了不良影响，包括精子计数减少、活力降低、形态异常增加、残留组蛋白、鱼精蛋白缺乏、精子 DNA 碎片化、睾丸抗氧化能力降低以及 RAGE 和 CML 蛋白水平升高。这些发现强调了 AGE 对男性生殖健康的负面影响，特别是在代谢紊乱的情况下，突出了 AGE/RAGE 轴在男性不育症中的关键作用，尤其是在西方饮食模式的情况下。
