FeO、FeO@CuO 和 WO 纳米粒子的简便合成：表征、结构测定及生物活性评价。

Facile synthesis of FeO, FeO@CuO and WO nanoparticles: characterization, structure determination and evaluation of their biological activity.

机构信息

Nanotechnology Research Center (NTRC), The British University in Egypt, El-Shorouk City, Suez Desert Road, P.O. Box 43, Cairo, 11837, Egypt.

Basic Science Department, Preparatory Year, University of Ha'il, 1560, Hail, Saudi Arabia.

出版信息

Sci Rep. 2024 Mar 13;14(1):6081. doi: 10.1038/s41598-024-55319-8.

DOI:10.1038/s41598-024-55319-8

PMID:38480834

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10937632/

Abstract

Due to their high specific surface area and its characteristic's functionalized nanomaterials have great potential in medical applications specialty, as an anticancer. Herein, functional nanoparticles (NPs) based on iron oxide FeO, iron oxide modified with copper oxide FeO@CuO, and tungsten oxide WO were facile synthesized for biomedical applications. The obtained nanomaterials have nanocrystal sizes of 35.5 nm for FeO, 7 nm for FeO@CuO, and 25.5 nm for WO. In addition to octahedral and square nanoplates for FeO, and WO respectively. Results revealed that FeO, FeO@CuO, and WO NPs showed remarked anticancer effects versus a safe effect on normal cells through cytotoxicity test using MTT-assay. Notably, synthesized NPs e.g. our result demonstrated that FeO@CuO exhibited the lowest IC value on the MCF-7 cancer cell line at about 8.876 µg/ml, compared to FeO was 12.87 µg/ml and WO was 9.211 µg/ml which indicate that the modification NPs FeO@CuO gave the highest antiproliferative effect against breast cancer. However, these NPs showed a safe mode toward the Vero normal cell line, where IC were monitored as 40.24 µg/ml for FeO, 21.13 µg/ml for FeO@CuO, and 25.41 µg/ml for WO NPs. For further evidence. The antiviral activity using virucidal and viral adsorption mechanisms gave practiced effect by viral adsorption mechanism and prevented the virus from replicating inside the cells. FeO@CuO and WO NPs showed a complete reduction in the viral load synergistic effect of combinations between the tested two materials copper oxide instead of iron oxide alone. Interestingly, the antimicrobial efficiency of FeO@CuO NPs, FeONPs, and WONPs was evaluated using E. coli, S. aureus, and C. albicans pathogens. The widest microbial inhibition zone (ca. 38.45 mm) was observed with 250 mg/ml of WO NPs against E. coli, whereas using 40 mg/ml of FeO@CuO NPS could form microbial inhibition zone ca. 32.86 mm against S. aureus. Nevertheless, C. albicans was relatively resistant to all examined NPs. The superior biomedical activities of these nanostructures might be due to their unique features and accepted evaluations.

摘要

由于其高比表面积和功能化特性，纳米材料在医学应用领域具有巨大的潜力，如作为抗癌药物。在此，基于氧化铁 FeO、氧化铁修饰的氧化铜 FeO@CuO 和氧化钨 WO 简便地合成了用于生物医学应用的功能纳米粒子（NPs）。所获得的纳米材料具有 35.5nm 的纳米晶尺寸 FeO、7nm 的 FeO@CuO 和 25.5nm 的 WO。此外，还分别具有八面体和正方形纳米板的 FeO 和 WO。结果表明，通过 MTT 分析的细胞毒性试验，FeO、FeO@CuO 和 WO NPs 对正常细胞表现出显著的抗癌作用，而对正常细胞的安全性高。值得注意的是，合成的 NPs，例如我们的结果表明，FeO@CuO 在 MCF-7 癌细胞系中的最低 IC 值约为 8.876µg/ml，而 FeO 为 12.87µg/ml，WO 为 9.211µg/ml，表明修饰 NPs FeO@CuO 对乳腺癌具有最高的抗增殖作用。然而，这些 NPs 对 Vero 正常细胞系表现出安全模式，IC 监测值分别为 FeO 为 40.24µg/ml、FeO@CuO 为 21.13µg/ml 和 WO NPs 为 25.41µg/ml。进一步的证据表明，抗病毒活性通过病毒吸附机制和阻止病毒在细胞内复制来实现病毒杀除和病毒吸附机制的协同作用。FeO@CuO 和 WO NPs 显示出协同作用，完全降低了病毒载量，这是两种测试材料氧化铜而不是氧化铁单独作用的结果。有趣的是，使用大肠杆菌、金黄色葡萄球菌和白色念珠菌病原体评估了 FeO@CuO NPs、FeONPs 和 WONPs 的抗菌效率。在 WO NPs 中，250mg/ml 的 WO NPs 对大肠杆菌的抑菌圈最宽（约 38.45mm），而 40mg/ml 的 FeO@CuO NPS 对金黄色葡萄球菌的抑菌圈约为 32.86mm。然而，所有检查的 NPs 对白色念珠菌的抵抗力相对较强。这些纳米结构具有优越的生物医学活性，可能是由于其独特的特性和公认的评估。