Mutran Sheila Cristina Almeida Neves, de Carvalho-Filho Paulo Roberto, Ribeiro Mara Eliane Soares, Faial Kelson do Carmo Freitas, Lima Rafael Rodrigues, D'Almeida Couto Roberta Souza
Department of Dentistry, School of Dentistry, Federal University of Pará, Belém, Brazil.
Department of Dentistry, School of Dentistry, University of São Paulo, São Paulo, Brazil.
Front Chem. 2024 Feb 28;12:1341769. doi: 10.3389/fchem.2024.1341769. eCollection 2024.
The use of natural products such as essential oils has been suggested due to their promising pharmacological effects and economic viability. This study aimed to determine hydrogenic potential (pH), titratable acidity (TA), and ion concentrations of five solutions containing essential oils (EO), when used as a EO-containing solutions, and evaluate ion concentrations, enamel surface loss, and morphology alterations in enamel. The pH, TA, calcium (Ca), potassium (K), and sodium (Na) concentrations of five EO-containing solutions were measured. Bovine enamel specimens were submitted to two daily 30-s immersions in artificial saliva, citric acid, distilled water, BaCloTea (Basil, Clove e Tea Tree), GeLaTeaPep (Geranium, Lavender, Tea Tree and Peppermint), EucaLem (Eucalyptus and Lemon), Cinnamon, or Spearmint solutions for 14 days. Ca, K, Na, and phosphorus (P) were quantified through ions chromatography, enamel surface loss was determined by profilometry, and surface morphology was qualitatively analyzed through scanning electron microscopy. Data were submitted to one-way ANOVA and Tukey ( < 0.05). The five EO-containing solutions presented significantly lower pH values than distilled water ( < 0.05). The GeLaTeaPep group presented a significantly higher TA value than BaCloTea ( < 0.05), which in turn showed a significantly higher TA value than the other solutions ( < 0.05). The distilled water presented significantly higher Ca, K, and Na concentrations than all EO-containing solutions ( < 0.05). The enamel exposed to EO-containing solutions showed lower Ca and P concentrations than artificial saliva (control) as well as significantly higher surface loss; however, the surface morphology was similar to the artificial saliva. EO-containing solutions have low pH, TA, and low concentrations of Ca, Na, and K. Moreover, enamel exposed to these solutions showed low Ca and P concentrations and slight surface loss without morphology alteration.
由于天然产物如香精油具有良好的药理作用和经济可行性，因此有人建议使用它们。本研究旨在确定五种含香精油（EO）溶液用作含EO溶液时的氢离子浓度（pH）、可滴定酸度（TA）和离子浓度，并评估离子浓度、牙釉质表面损失和牙釉质形态变化。测量了五种含EO溶液的pH、TA、钙（Ca）、钾（K）和钠（Na）浓度。将牛牙釉质标本每天两次在人工唾液、柠檬酸、蒸馏水、BaCloTea（罗勒、丁香和茶树）、GeLaTeaPep（天竺葵、薰衣草、茶树和薄荷）、桉柠（桉树和柠檬）、肉桂或留兰香溶液中浸泡30秒，持续14天。通过离子色谱法定量分析Ca、K、Na和磷（P），通过轮廓测定法确定牙釉质表面损失，并通过扫描电子显微镜对表面形态进行定性分析。数据进行单因素方差分析和Tukey检验（<0.05）。五种含EO溶液的pH值明显低于蒸馏水（<0.05）。GeLaTeaPep组的TA值明显高于BaCloTea组（<0.05），而BaCloTea组的TA值又明显高于其他溶液（<0.05）。蒸馏水的Ca、K和Na浓度明显高于所有含EO溶液（<0.05）。暴露于含EO溶液的牙釉质显示出比人工唾液（对照）更低的Ca和P浓度以及明显更高的表面损失；然而，表面形态与人工唾液相似。含EO溶液具有低pH、低TA以及低浓度的Ca、Na和K。此外，暴露于这些溶液的牙釉质显示出低Ca和P浓度以及轻微的表面损失，且无形态改变。
