酵母来源的β-(1→3)(1→6)-D-葡聚糖对自然杀伤（NK）细胞和乳腺癌细胞系在 2D 和 3D 培养中的作用。

Role of beta-(1→3)(1→6)-D-glucan derived from yeast on natural killer (NK) cells and breast cancer cell lines in 2D and 3D cultures.

机构信息

Berlin Institute of Health at Charité - Universitätsmedizin Berlin, Charitéplatz 1, Berlin, 10117, Germany.

Competence Center of Immuno-Oncology and Translational Cell Therapy (KITZ), Department of Hematology, Oncology and Tumor Immunology, CCM, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Charitéplatz 1, Berlin, 10117, Germany.

出版信息

BMC Cancer. 2024 Mar 14;24(1):339. doi: 10.1186/s12885-024-11979-3.

DOI:10.1186/s12885-024-11979-3

PMID:38486205

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10938759/

Abstract

BACKGROUND

Beta-(1,3)(1,6)-D-glucan is a complex polysaccharide, which is found in the cell wall of various fungi, yeasts, bacteria, algae, barley, and oats and has immunomodulatory, anticancer and antiviral effects. In the present study, we investigated the effect of beta-(1,3)(1,6)-D-glucan derived from yeast on the proliferation of primary NK cells and breast cancer cell lines in 2D and 3D models, and on the cytotoxicity of primary NK cells against breast cancer cell lines in 2D and 3D models.

METHODS

In this study, we investigated the effects of different concentrations of yeast-derived beta-(1→3)(1→6)-D-glucan on the proliferation and cytotoxicity of human NK cells and breast cancer cell lines in 2D and 3D models using the XTT cell proliferation assay and the CellTiter-Glo® 2.0 assay to determine the cytotoxicity of human NK cells on breast cancer cell lines in 2D and 3D models.

RESULTS

We found that the co-incubation of NK cells with beta-glucan in the absence of IL2 at 48 h significantly increased the proliferation of NK cells, whereas the co-incubation of NK cells with beta-glucan in the presence of IL2 (70 U/ml) increased the proliferation of NK cells but not significantly. Moreover, beta-glucan significantly inhibited the proliferation of breast cancer cell lines in 2D model and induced a weak, non-significant growth inhibitory effect on breast cancer multicellular tumor spheroids (3D). In addition, the cytotoxicity of NK cells against breast cancer cell lines was examined in 2D and 3D models, and beta-glucan significantly increased the cytotoxicity of NK cells against MCF-7 (in 2D).

CONCLUSIONS

Yeast derived beta-(1,3)(1,6)-D-glucan could contribute to the treatment of cancer by enhancing NK cell immune response as well as contributing to inhibition of breast cancer cell growth.

摘要

背景

β-（1,3）（1,6）-D-葡聚糖是一种复杂的多糖，存在于各种真菌、酵母、细菌、藻类、大麦和燕麦的细胞壁中，具有免疫调节、抗癌和抗病毒作用。在本研究中，我们研究了酵母来源的β-（1,3）（1,6）-D-葡聚糖对 2D 和 3D 模型中原代 NK 细胞和乳腺癌细胞系增殖的影响，以及对 2D 和 3D 模型中原代 NK 细胞对乳腺癌细胞系细胞毒性的影响。

方法

在这项研究中，我们使用 XTT 细胞增殖测定法和 CellTiter-Glo® 2.0 测定法研究了不同浓度的酵母来源的β-（1→3）（1→6）-D-葡聚糖对 2D 和 3D 模型中人类 NK 细胞和乳腺癌细胞系增殖和细胞毒性的影响，以确定 2D 和 3D 模型中人类 NK 细胞对乳腺癌细胞系的细胞毒性。

结果

我们发现，NK 细胞与β-葡聚糖在没有 IL2 的情况下共孵育 48 小时可显著增加 NK 细胞的增殖，而 NK 细胞与含有 IL2（70 U/ml）的β-葡聚糖共孵育可增加 NK 细胞的增殖，但不显著。此外，β-葡聚糖显著抑制 2D 模型中乳腺癌细胞系的增殖，并对乳腺癌多细胞肿瘤球体（3D）产生较弱的、非显著的生长抑制作用。此外，在 2D 和 3D 模型中检查了 NK 细胞对乳腺癌细胞系的细胞毒性，β-葡聚糖显著增加了 NK 细胞对 MCF-7（在 2D 中）的细胞毒性。