Peng Chenjia, Wang Ying, Zhang Hengbo, Chen Ping
School of Mathematics and Computational Science, Hunan First Normal University, Changsha, 410205, PR China.
The National & Local Joint Engineering Laboratory of Animal Peptide Drug Development, College of Life Sciences, Hunan Normal University, Changsha, 410081, PR China.
Heliyon. 2024 Feb 27;10(5):e26798. doi: 10.1016/j.heliyon.2024.e26798. eCollection 2024 Mar 15.
Accumulating evidence highlighted the important roles of platelets in the prognosis and progression of various tumors. Nevertheless, the role of platelet-related genes (PRGs) in HCC remains limited. In this work, 92 differentially expressed PRGs were described in HCC using TCGA and ICGC databases. Then, based on the different expressions of PRGs, we explored two subtypes and developed the PRGs prognostic signature in HCC. The PRGs signature was an independent prognosis factor associated with immune cell infiltration in HCC. Furthermore, two external validation sets verified the expression and prognosis of the PRGs signature gene in HCC. Finally, scRNA-seq analysis demonstrated that the signature genes (CENPE and KIF2C) were mainly expressed in cycling T cells and prolif-TAMs. Enrichment analysis showed that CENPE and KIF2C regulated the cell cycle and p53 pathways in these cells. In conclusion, this study builds the PRGs-related risk signature of HCC and reveals the potential mechanism by which these signature genes regulate the immune microenvironment in HCC.
越来越多的证据表明血小板在各种肿瘤的预后和进展中起着重要作用。然而，血小板相关基因（PRGs）在肝癌中的作用仍然有限。在这项研究中，利用TCGA和ICGC数据库描述了肝癌中92个差异表达的PRGs。然后，基于PRGs的不同表达，我们探索了两种亚型并建立了肝癌的PRGs预后特征。PRGs特征是与肝癌免疫细胞浸润相关的独立预后因素。此外，两个外部验证集验证了PRGs特征基因在肝癌中的表达和预后。最后，单细胞RNA测序分析表明，特征基因（CENPE和KIF2C）主要在循环T细胞和增殖性肿瘤相关巨噬细胞中表达。富集分析表明，CENPE和KIF2C在这些细胞中调节细胞周期和p53通路。总之，本研究构建了肝癌的PRGs相关风险特征，并揭示了这些特征基因调节肝癌免疫微环境的潜在机制。
