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从帕斯特马干酪中分离出的肠球菌的基因型多样性和拮抗活性。

Genotypic diversity and antagonistic activities of enterococci isolated from pastırma.

作者信息

Ertekin Özlem, Kaban Güzin, Kaya Mükerrem

机构信息

Department of Nutrition and Dietetics, Faculty of Health Sciences, Munzur University, 62000 Tunceli, Turkey.

Department of Food Engineering, Faculty of Agriculture, Atatürk University, 25240 Erzurum, Turkey.

出版信息

J Food Sci Technol. 2024 May;61(5):983-989. doi: 10.1007/s13197-023-05895-w. Epub 2023 Nov 27.

Abstract

The biodiversity of enterococci from pastırma (a traditional Turkish dry-cured meat product) by genotypic identification and the antagonistic activities of strains were investigated. Pastırma samples taken from 20 different small-scale factories were subjected to microbiological and physicochemical analysis. A hundred enterococci isolates were identified by 16S rRNA gene sequence analysis. To determine antagonistic activity of strains, and were used. The lactic acid bacteria and counts were ≥ 6 log cfu/g in 55% of the samples and 75% of the samples, respectively. Enterobacteriaceae was generally below the detectable level (< 2 log cfu/g). The enterococci count was higher than 6 log cfu/g in 30-35% of the samples, depending on the medium used. The enterococci isolates (100 isolates) were identified as (80 strains), (19 strains) and (1 strain) in genotypic identification. The nine strains showed antagonistic activity against in the well diffusion test. In contrast, in the same antagonistic activity test, all of the strains had no antagonistic activity against . Further studies could be planned to characterize strains that show antagonistic activity against .

摘要

通过基因型鉴定对来自土耳其传统干腌肉制品“帕斯特马”的肠球菌的生物多样性以及菌株的拮抗活性进行了研究。从20个不同的小型工厂采集的帕斯特马样品进行了微生物学和理化分析。通过16S rRNA基因序列分析鉴定了100株肠球菌分离株。为了确定菌株的拮抗活性,使用了[具体物质未给出]。乳酸菌和[具体物质未给出]的计数在55%的样品中分别≥6 log cfu/g和在75%的样品中分别≥6 log cfu/g。肠杆菌科通常低于可检测水平(<2 log cfu/g)。根据使用的培养基不同,肠球菌计数在30% - 35%的样品中高于6 log cfu/g。在基因型鉴定中,100株肠球菌分离株被鉴定为粪肠球菌(80株)、屎肠球菌(19株)和鸟肠球菌(1株)。在琼脂扩散试验中,9株粪肠球菌菌株对[具体物质未给出]表现出拮抗活性。相反,在相同的拮抗活性试验中,所有菌株对[具体物质未给出]均无拮抗活性。可以计划进一步研究来表征对[具体物质未给出]表现出拮抗活性的粪肠球菌菌株。

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The role and application of enterococci in food and health.肠球菌在食品与健康中的作用及应用。
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